Anticorps Monoclonal anti-GPT/ALT1

GPT/ALT1 Monoclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

CloneNo.

5F6B5

N° de cat : 67531-1-Ig

Synonymes

AAT1, Alanine aminotransferase 1, ALAT1, ALT1, GPT, GPT 1, GPT/ALT1, GPT1

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Galerie de données de validation

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  WB analysis of rat liver using 67531-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 67531-1-Ig (GPT/ALT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67531-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67531-1-Ig (GPT/ALT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 67531-1-Ig

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 67531-1-Ig (GPT/ALT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig heart using 67531-1-Ig

  pig heart tissue were subjected to SDS PAGE followed by western blot with 67531-1-Ig (GPT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig liver using 67531-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 67531-1-Ig (GPT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 67531-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GPT antibody (67531-1-Ig, Clone: 5F6B5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: lait humain, tissu cardiaque de lapin, tissu cardiaque de porc, tissu hépatique de porc, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67531-1-Ig cible GPT/ALT1 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GPT/ALT1 Protéine recombinante Ag10453
• Nom complet: glutamic-pyruvate transaminase (alanine aminotransferase)
• Masse moléculaire calculée: 496 aa, 55 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC018207
• Symbole du gène: GPT
• Identification du gène (NCBI): 2875
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GPT, also known as ALT1 (glutamate-pyruvate transaminase 1), catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.