Anticorps Polyclonal de lapin anti-GPX1

GPX1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 29329-1-AP

Synonymes

glutathione peroxidase 1, GPx 1, GPX1, GSHPx 1, GSHPX1

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Galerie de données de validation

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  WB analysis using 29329-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29329-1-AP (GPX1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 29329-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29329-1-AP (GPX1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 29329-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29329-1-AP (GPX1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules SMMC-7721, cellules THP-1, tissu rénal de rat, tissu rénal de souris

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IF: See 1 publications below

Informations sur le produit

29329-1-AP cible GPX1 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GPX1 Protéine recombinante Ag31101
• Nom complet: glutathione peroxidase 1
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC000742
• Symbole du gène: GPX1
• Identification du gène (NCBI): 2876
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Gpx protein, known as selenoprotein, consists selenium in its catalytic site. Gpx1 is an abundant isoform, which involves in scavenging endogenous ROS using electron provided by reduced glutathione and ubiquitously expressed the cytoplasm and mitochondria of all cell types. (PMID: 30632027)

Publications

Species	Application	Title
mouse	WB	Mol Ther; CircRNA Samd4 induces cardiac repair after myocardial infarction by blocking mitochondria-derived ROS output.; Authors - Hao Zheng
human,mouse	WB,IF	Cell Death Discov; Allicin promotes functional recovery in ischemic stroke via glutathione peroxidase-1 activation of Src-Akt-Erk; Authors - Fei Zhuang ; KD Validated
mouse	WB	Molecules; Polygonatum cyrtonema Hua Polysaccharides Protect BV2 Microglia Relief Oxidative Stress and Ferroptosis by Regulating NRF2/HO-1 Pathway; Authors - Jiayu Li
rat	WB	Pharmaceuticals (Basel); Adrenomedullin Improves Cardiac Remodeling and Function in Obese Rats with Hypertension.; Authors - Pei Qian
rat	WB	Acta Diabetol; Adrenomedullin ameliorates palmitic acid-induced insulin resistance through PI3K/Akt pathway in adipocytes.; Authors - Hang-Bing Dai
mouse	WB	bioRxiv; WDR23 mediates NRF2 proteostasis and cytoprotective capacity in the hippocampus; Authors - Jiahui Liu