Anticorps Polyclonal de lapin anti-GPX4

• Phare
• Validé par KD/KO

GPX4 Polyclonal Antibody for WB, IF-P, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF-P, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications(130)

N° de cat : 30388-1-AP

Synonymes

EC:1.11.1.12, EC:1.11.1.9, GPx 4, GPx-4, GSHPx 4

---

Galerie de données de validation

• 

  WB analysis using 30388-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30388-1-AP (GPX4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 30388-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30388-1-AP (GPX4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 30388-1-AP

  WB result of GPX4 antibody (30388-1-AP; 1:2400; incubated at room temperature for 1.5 hours) with sh-Control and sh-GPX4 transfected HEK-293 cells.

• 

  IP experiment of rat testis using 30388-1-AP

  IP result of anti-GPX4 (IP:30388-1-AP, 4ug; Detection:30388-1-AP 1:3000) with rat testis tissue lysate 1880 ug.

• 

  IF Staining of rat testis using 30388-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat testis tissue using GPX4 antibody (30388-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat testis using 30388-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat testis tissue using GPX4 antibody (30388-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HeLa using 30388-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human GPX4 (30388-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en WB: cellules HCT 116, cellules HEK-293, cellules HL-60, cellules Jurkat, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: tissu testiculaire de rat,
• Résultats positifs en IF-P: tissu testiculaire de rat,
• Résultats positifs en FC (Intra): cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 117 publications below
• IHC: See 21 publications below
• IF: See 20 publications below
• IP: See 1 publications below

Informations sur le produit

30388-1-AP cible GPX4 dans les applications de WB, IHC, IF-P, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GPX4 Protéine recombinante Ag30650
• Nom complet: glutathione peroxidase 4 (phospholipid hydroperoxidase)
• Poids moléculaire observé: 18-23 kDa
• Numéro d’acquisition GenBank: BC021567
• Symbole du gène: GPX4
• Identification du gène (NCBI): 2879
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GPX4 (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial) protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility.It has two isforms about 20KDa and 22KDa, respectively. GPX4 is a monomer,but it has a tendency to form higher mass oligomers (PMID:17630701). It presents primarily in testis.

Publications

Species	Application	Title
rat,mouse	WB,IHC,IF	Bioact Mater; A two-pronged approach to inhibit ferroptosis of MSCs caused by the iron overload in postmenopausal osteoporosis and promote osseointegration of titanium implant; Authors - Yulu Yang
rat	WB,IHC	Nat Commun; Injectable ECM-mimetic dynamic hydrogels abolish ferroptosis-induced post-discectomy herniation through delivering nucleus pulposus progenitor cell-derived exosomes; Authors - Wenkai Wang
mouse	WB	J Hazard Mater; Investigating the potential risk of cadmium exposure on seizure severity and anxiety-like behaviors through the ferroptosis pathway in epileptic mice: An integrated multi-omics approach; Authors - Yuanjin Chang
mouse,human	WB,IF	Sci Adv; In situ implantable DNA hydrogel for diagnosis and therapy of postoperative rehemorrhage following intracerebral hemorrhage surgery; Authors - Wenyan Yu
mouse,human	WB,IHC,IF	J Nanobiotechnology; Eriodictyol-cisplatin coated nanomedicine synergistically promote osteosarcoma cells ferroptosis and chemosensitivity; Authors - Zili Lin
human	WB,IHC	Adv Healthc Mater; Fe3 O4 -Incorporated Metal-Organic Framework for Chemo/Ferroptosis Synergistic Anti-Tumor via the Enhanced Chemodynamic Therapy; Authors - Yuliang Xu

{{ptg:RelatedPrimaryAntibodies}}