Anticorps Polyclonal de lapin anti-GPX7

• Phare
• Validé par KD/KO

GPX7 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (3)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(17)

N° de cat : 13501-1-AP

Synonymes

CL683, EC:1.11.1.9, GPx 7, GPX6, GPx-7

---

Galerie de données de validation

• 

  WB analysis of human liver using 13501-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13501-1-AP (GPX7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13501-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13501-1-AP (GPX7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Transfected HEK-293 using 13501-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 13501-1-AP (GPX7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 13501-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13501-1-AP (GPX7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 13501-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13501-1-AP (GPX7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 13501-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13501-1-AP (GPX7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 13501-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 13501-1-AP (GPX7 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 13501-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 13501-1-AP (GPX7 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 13501-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 13501-1-AP (GPX7 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 13501-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 13501-1-AP (GPX7 antibody) at dilution of 1:50 (under 40x lens).

---

Applications testées

• Résultats positifs en WB: tissu hépatique humain, cellules HEK-293 transfectées, tissu cérébral humain, tissu placentaire humain
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 14 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Informations sur le produit

13501-1-AP cible GPX7 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GPX7 Protéine recombinante Ag4328
• Nom complet: glutathione peroxidase 7
• Masse moléculaire calculée: 187 aa, 21 kDa
• Poids moléculaire observé: 18-21 kDa
• Numéro d’acquisition GenBank: BC032788
• Symbole du gène: GPX7
• Identification du gène (NCBI): 2882
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GPX7(Glutathione peroxidase 7) is also named as GPX6,CL683,GSHPx-7 and belongs to the glutathione peroxidase family.It shares approximately 45% amino acid sequence with other GPX family members.Because GPX7 expression protected cells from acidic bile acidinduced DNA damage, it may protect oesophageal epithelial cells from acidic bile acid-induced apoptosis(PMID:22157330). This antibody can ve detected as 18-21kDa due to signal peptide splicing.

Publications

Species	Application	Title
human	WB	Gut; Glutathione peroxidase 7 protects against oxidative DNA damage in oesophageal cells.; Authors - Peng Dunfa D
human	WB	Gut; Glutathione peroxidase 7 has potential tumour suppressor functions that are silenced by location-specific methylation in oesophageal adenocarcinoma.; Authors - Peng Dunfa D
mouse	WB	EMBO Mol Med; Deficiency of NPGPx, an oxidative stress sensor, leads to obesity in mice and human.; Authors - Chang Yi-Cheng YC
human	IHC	Oncogene; Abrogation of glutathione peroxidase-1 drives EMT and chemoresistance in pancreatic cancer by activating ROS-mediated Akt/GSK3β/Snail signaling.; Authors - Qingcai Meng
human	WB	Carcinogenesis; Loss of glutathione peroxidase 7 promotes TNF-α-induced NF-κB activation in Barrett's carcinogenesi.; Authors - Dun-Fa Peng ; KD Validated
	WB	Free Radic Biol Med; GPx8 peroxidase prevents leakage of H2O2 from the endoplasmic reticulum.; Authors - Thomas Ramming

{{ptg:RelatedPrimaryAntibodies}}