Anticorps Polyclonal de lapin anti-GPX8

GPX8 Polyclonal Antibody for IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

5

Avis (1)

N° de cat : 16846-1-AP

Synonymes

GPx 8, GPX8, GSHPx 8, UNQ847

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Galerie de données de validation

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  WB analysis of U-251 using 16846-1-AP

  U-251 cells were subjected to SDS PAGE followed by western blot with 16846-1-AP (GPX8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 16846-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16846-1-AP (GPX8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 16846-1-AP

  IP Result of anti-GPX8 (IP:16846-1-AP, 4ug; Detection:16846-1-AP 1:300) with HEK-293 cells lysate 3000ug.

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  IHC staining of human stomach cancer using 16846-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16846-1-AP (GPX8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 16846-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16846-1-AP (GPX8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules U-251, cellules HEK-293
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

16846-1-AP cible GPX8 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GPX8 Protéine recombinante Ag9930
• Nom complet: glutathione peroxidase 8 (putative)
• Masse moléculaire calculée: 209 aa, 24 kDa
• Poids moléculaire observé: 24 kDa
• Numéro d’acquisition GenBank: BC029424
• Symbole du gène: GPX8
• Identification du gène (NCBI): 493869
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GPX8(Probable glutathione peroxidase 8) is also named as GSHPx-8 and belongs to the glutathione peroxidase family. Glutathione peroxidase (GPx) reduces hydroperoxides, including hydrogen peroxides, in the presence of reduced glutathione as a means of protecting organisms from oxidative damage. Several GPx isozymes have been identified in animal cells and these have been classified into different groups according to their cellular location and substrate specificity. In humans, eight types of GPx have been identified from GPx1 to GPx8. The full length GPX8 has 209 amino acids and the molecular weight is 24 kDa.

Publications

Species	Application	Title
human	WB	J Med Virol; SARS-CoV-2 nucleocapsid protein enhances the level of mitochondrial reactive oxygen species; Authors - Haiyun Yu
rat	WB,IF	Free Radic Biol Med; ER-resident antioxidative GPx7 and GPx8 enzyme isoforms protect insulin-secreting INS-1E β-cells against lipotoxicity by improving the ER antioxidative capacity.; Authors - Ilir Mehmeti
human	IHC	Medicina (Kaunas); Chosen Antioxidant Enzymes GPx4 and GPx8 in Human Colorectal Carcinoma: Study of the Slovak Population.; Authors - Andriana Pavliuk-Karachevtseva
human	WB,IHC	MedComm (2020); Glutathione peroxidase 8 expression on cancer cells and cancer-associated fibroblasts facilitates lung cancer metastasis; Authors - Yu‐Lian Xu ; KD Validated
rat	WB	FASEB J; Luminal H(2) O(2) promotes ER Ca(2+) dysregulation and toxicity of palmitate in insulin-secreting INS-1E cells; Authors - Sarah Sharifi

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Andrea (Verified Customer) (01-23-2023)

Sharp and strong signal at 24 kDa with clear background.

• Applications: Western Blot