Anticorps Polyclonal de lapin anti-GRAMD1B

• Phare
• Validé par KD/KO

GRAMD1B Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(10)

N° de cat : 24905-1-AP

Synonymes

GRAM domain containing 1B, GRAM domain-containing protein 1B, KIAA1201, Protein Aster-B, UNQ3032/PRO9834

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Galerie de données de validation

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  WB analysis of A549 using 24905-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 24905-1-AP (GRAMD1B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 24905-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24905-1-AP (GRAMD1B Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 24905-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24905-1-AP (GRAMD1B Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 24905-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed A549 cells using 24905-1-AP (GRAMD1B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of A549 using 24905-1-AP

  1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human GRAMD1B (24905-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A549
• Résultats positifs en IHC: tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A549
• Résultats positifs en FC (Intra): cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 8 publications below
• IF: See 5 publications below

Informations sur le produit

24905-1-AP cible GRAMD1B dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GRAMD1B Protéine recombinante Ag20670
• Nom complet: GRAM domain containing 1B
• Masse moléculaire calculée: 738 aa, 85 kDa
• Poids moléculaire observé: 85 kDa
• Numéro d’acquisition GenBank: BC107480
• Symbole du gène: GRAMD1B
• Identification du gène (NCBI): 57476
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GRAMD1B, also named as KIAA1201, contains a transmembrane region and two domains of known function; the GRAM domain and a VASt domain. It is predicted to localize in the nucleus, supported by several nuclear transport signals and nuclearly associated motifs. This highly conserved gene is found in a variety of vertebrates and invertebrates, however, it is not found in bacteria or fungi. There're some isoforms with MW 50 kDa and 81-87 kDa. With phosphor modification, it's about 90-110 kDa in WB detection.

Publications

Species	Application	Title
human	IF	Nat Commun; GRAMD1B is a regulator of lipid homeostasis, autophagic flux and phosphorylated tau; Authors - Diana Acosta Ingram
mouse	WB	Mol Metab; Aster-B coordinates with Arf1 to regulate mitochondrial cholesterol transport.; Authors - John-Paul Andersen
human	WB,IF	J Lipid Res; ASTER-B regulates mitochondrial carotenoid transport and homeostasis; Authors - Sepalika Bandara ; KD Validated
human	WB	Noncoding RNA; MiR-4646-5p Acts as a Tumor-Suppressive Factor in Triple Negative Breast Cancer and Targets the Cholesterol Transport Protein GRAMD1B; Authors - Katharina Jonas
human	WB,IF	Exp Eye Res; Imaging Macular Carotenoids and Their Related Proteins in the Human Retina with Confocal Resonance Raman and Fluorescence Microscopy; Authors - Binxing Li
human	WB,IF	bioRxiv; Intracellular Retention of Estradiol is Augmented by GRAM Domain Containing Protein ASTER-B in Breast Cancer Cells; Authors - Hyung Bum Kim ; KD Validated

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