Anticorps Monoclonal anti-GRASP65,GORASP1

GRASP65,GORASP1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(4)

CloneNo.

1B2C10

N° de cat : 66651-1-Ig

Synonymes

GORASP1, 1B2C10, Golgi phosphoprotein 5, Golgi reassembly-stacking protein 1, Golgi reassembly-stacking protein of 65 kDa

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Galerie de données de validation

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  WB analysis using 66651-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66651-1-Ig (GRASP65,GORASP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 66651-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66651-1-Ig (GRASP65,GORASP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 66651-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66651-1-Ig (GRASP65,GORASP1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 66651-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66651-1-Ig (GRASP65,GORASP1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 66651-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66651-1-Ig (GRASP65,GORASP1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 66651-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66651-1-Ig (GRASP65,GORASP1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SK-BR-3 using 66651-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed SK-BR-3 cells using GRASP65,GORASP1 antibody (66651-1-Ig, Clone: 1B2C10 ) at dilution of 1:3876 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HSC-T6, cellules Jurkat, cellules MCF-7
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu de cancer de l'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: SK-BR-3 cells,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:1938-1:7752

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below

Informations sur le produit

66651-1-Ig cible GRASP65,GORASP1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GRASP65,GORASP1 Protéine recombinante Ag1054
• Nom complet: golgi reassembly stacking protein 1, 65kDa
• Masse moléculaire calculée: 46 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: BC008928
• Symbole du gène: GRASP65
• Identification du gène (NCBI): 64689
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Golgi reassembly-stacking protein 1 is a protein that in humans is encoded by the GORASP1 gene. The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum.

Publications

Species	Application	Title
human	WB	Front Cell Dev Biol; Autophagy-Based Unconventional Secretory for AIM2 Inflammasome Drives DNA Damage Resistance During Intervertebral Disc Degeneration.; Authors - Shuai Li
human	WB	Mol Biol Cell; Hydrogen peroxide induces Arl1 degradation and impairs Golgi-mediated trafficking.; Authors - Stephen C Ireland
human	WB	J Cell Biochem; Golgi apparatus fragmentation participates in oxidized low-density lipoprotein-induced endothelial cell injury.; Authors - Guang Ji
human	WB	Cells; Discovery of Cell Number-Interstitial Fluid Volume (CIF) Ratio Reveals Secretory Autophagy Pathway to Supply eHsp90α for Wound Healing; Authors - Cheng Chang ; KO Validated

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