Anticorps Polyclonal de lapin anti-GRB2

• Phare
• Validé par KD/KO

GRB2 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

13

N° de cat : 10254-2-AP

Synonymes

Adapter protein GRB2, ASH, EGFRBP GRB2, GRB2, Grb3 3, MST084, MSTP084, Protein Ash, SH2/SH3 adapter GRB2

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Galerie de données de validation

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  WB analysis of HEK-293 using 10254-2-AP

  WB result of GRB2 antibody (10254-2-AP; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-GRB2 transfected HEK-293 cells.

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  WB analysis of HEK-293 using 10254-2-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10254-2-AP (GRB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 10254-2-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10254-2-AP (GRB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 10254-2-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10254-2-AP (GRB2 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

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  WB analysis of rat spleen using 10254-2-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 10254-2-AP (GRB2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 10254-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10254-2-AP (GRB2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 10254-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10254-2-AP (GRB2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 10254-2-AP

  Immunofluorescent analysis of HEK-293 cells using 10254-2-AP (GRB2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules A431, tissu cérébral de rat, tissu splénique de rat
• Résultats positifs en IHC: tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 10 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Informations sur le produit

10254-2-AP cible GRB2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GRB2 Protéine recombinante Ag0395
• Nom complet: growth factor receptor-bound protein 2
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 25-28 kDa
• Numéro d’acquisition GenBank: BC000631
• Symbole du gène: GRB2
• Identification du gène (NCBI): 2885
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GRB2 (growth factor receptor-bound protein 2) binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. N-SH3 domain of Grb2 was involved in the protein vesicular localization including amyloid-beta protein precursor. Involvement of GRB2 in Ras-signaling pathway has been reported, recent finding show that GRB2 may also play a complex role in T and B-cell antigen receptor signaling.

Publications

Species	Application	Title
human	IHC,WB	Mol Ther Nucleic Acids; ZEB1-Mediated Transcriptional Upregulation of circWWC3 Promotes Breast Cancer Progression through Activating Ras Signaling Pathway.; Authors - Lingjiao Meng
human	WB	Anal Chem; Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.; Authors - Hongwei Chu
human	IHC	Cancer Lett; Deubiquitinase PSMD14 enhances hepatocellular carcinoma growth and metastasis by stabilizing GRB2.; Authors - Jie Lv
human	WB,IHC	Oncotarget; mir-329 restricts tumor growth by targeting grb2 in pancreatic cancer.; Authors - Xinjing Wang
		Cancer Lett; Melittin inhibits tumor growth and decreases resistance to gemcitabine by downregulating cholesterol pathway gene CLU in pancreatic ductal adenocarcinoma.; Authors - Xinjing Wang
human	WB	Cancer Sci; In vivo CRISPR screens identify RhoV as a pro-metastasis factor of triple-negative breast cancer; Authors - Ming-Liang Jin