Anticorps Polyclonal de lapin anti-GRIA1

GRIA1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 25012-1-AP

Synonymes

GluA1, GLUH1, GluR 1, GluR A, GluR K1, GLUR1, GLURA, Glutamate receptor 1, GRIA1, HBGR1

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Galerie de données de validation

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  WB analysis using 25012-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25012-1-AP (GRIA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 25012-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 25012-1-AP (GRIA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 25012-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25012-1-AP (GRIA1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 25012-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25012-1-AP (GRIA1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 25012-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25012-1-AP (GRIA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu de cervelet de souris, tissu de cervelet de rat
• Résultats positifs en IHC: tissu cérébral humain, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

25012-1-AP cible GRIA1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GRIA1 Protéine recombinante Ag21502
• Nom complet: glutamate receptor, ionotropic, AMPA 1
• Masse moléculaire calculée: 906 aa, 102 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC111734
• Symbole du gène: GRIA1
• Identification du gène (NCBI): 2890
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. This gene belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors.

Publications

Species	Application	Title
rat	WB,IF	Food Chem Toxicol; Melatonin attenuates BDE-209-caused spatial memory deficits in juvenile rats through NMDAR-CaMKⅡγ-mediated synapse-to-nucleus signaling; Authors - Jinghua Shen
rat	IHC	Biomed Chromatogr; Revealing the optimal traditional processing methods and its protective effects against febrile seizures of Arisaema cum bile; Authors - Meng-Meng Zhang
mouse	WB	Cells; Treadmill Exercise Facilitates Synaptic Plasticity in APP/PS1 Mice by Regulating Hippocampal AMPAR Activity; Authors - Laikang Yu
mouse	WB	Behav Pharmacol; Hesperidin produces antidepressant effects by activating AMPA receptor: enhancing synaptic proteins to promote hippocampal neuronal activities; Authors - Bo Pang
mouse	WB	J Ethnopharmacol; Iridoids from Gardeniae fructus ameliorates depression by enhancing synaptic plasticity via AMPA receptor-mTOR signaling; Authors - Baomei Xia

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