Anticorps Monoclonal anti-GRIA1

GRIA1 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

CloneNo.

1F4A1

Avis (2)

N° de cat : 67642-1-Ig

Synonymes

GluA1, GLUH1, GluR 1, GluR A, GluR K1, GLUR1, GLURA, Glutamate receptor 1, GRIA1, HBGR1

---

Galerie de données de validation

• 

  WB analysis using 67642-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67642-1-Ig (GRIA1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig spinal cord using 67642-1-Ig

  pig spinal cord tissue were subjected to SDS PAGE followed by western blot with 67642-1-Ig (GRIA1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig cerebellum using 67642-1-Ig

  pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 67642-1-Ig (GRIA1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 67642-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67642-1-Ig (GRIA1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 67642-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67642-1-Ig (GRIA1 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 67642-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67642-1-Ig (GRIA1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 67642-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GRIA1 antibody (67642-1-Ig, Clone: 1F4A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse brain using 67642-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GRIA1 antibody (67642-1-Ig, Clone: 1F4A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

---

Applications testées

• Résultats positifs en WB: tissu cérébral de rat, tissu cérébral de lapin, tissu cérébral de porc, tissu cérébral de souris, tissu de cervelet de porc, tissu de cervelet de rat, tissu de cervelet de souris, tissu de moelle épinière de porc
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:20000-1:100000
• Immunohistochimie (IHC): IHC : 1:5000-1:20000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

67642-1-Ig cible GRIA1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GRIA1 Protéine recombinante Ag28932
• Nom complet: glutamate receptor, ionotropic, AMPA 1
• Masse moléculaire calculée: 906 aa, 102 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC111734
• Symbole du gène: GRIA1
• Identification du gène (NCBI): 2890
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	CNS Neurosci Ther; Integrin α3 is required for high-frequency repetitive transcranial magnetic stimulation-induced glutamatergic synaptic transmission in mice with ischemia; Authors - Li Liu
rat	WB	Exp Neurol; Long-term variable photoperiod exposure impairs the mPFC and induces anxiety and depression-like behavior in male wistar rats; Authors - YuanYuan Hou
mouse	WB	Phytother Res; Rapid antidepressant-like effect of Fructus Aurantii depends on cAMP-response element binding protein/Brain-derived neurotrophic facto by mediating synaptic transmission.; Authors - Lei Wu
rat	WB	J Psychiatr Res; Hippocampal AMPA receptors mediate the impairment of spatial learning and memory in prenatally stressed offspring rats.; Authors - Dan Yao
mouse	WB	Cell Biosci; Amelioration of olfactory dysfunction in a mouse model of Parkinson's disease via enhancing GABAergic signaling; Authors - Xing-Yang Liu
mouse	WB,IF,IP	CNS Neurosci Ther; Inhibition of ANXA2 activity attenuates epileptic susceptibility and GluA1 phosphorylation; Authors - Limin Ma

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Pegah (Verified Customer) (02-03-2023)

I used a concentration of 1:500 of GRIA1 Monoclonal antibody (67642-1-Ig) for paraformaldehyde fixed mouse brain tissue with 40um diameter.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain

GRIA1 Antibody Immunofluorescence validation (1:500 dilution) in Mouse brain (Cat no:67642-1-Ig)

FH

Lisa (Verified Customer) (10-04-2021)

The antibody signal is very strong and clean. Highly recommended.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: hiPSCs-derived neurons