Anticorps Polyclonal de lapin anti-GPRIN1

• Phare
• Validé par KD/KO

GPRIN1 Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 13771-1-AP

Synonymes

GPRIN1, GRIN1, KIAA1893

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Galerie de données de validation

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  WB analysis of mouse brain using 13771-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13771-1-AP (GPRIN1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 13771-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13771-1-AP (GPRIN1 antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 13771-1-AP

  IP Result of anti-GPRIN1 (IP:13771-1-AP, 3ug; Detection:13771-1-AP 1:500) with mouse brain tissue lysate 3600ug.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris
• Résultats positifs en IP: tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

13771-1-AP cible GPRIN1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GPRIN1 Protéine recombinante Ag4754
• Nom complet: G protein regulated inducer of neurite outgrowth 1
• Masse moléculaire calculée: 1008 aa, 102 kDa
• Poids moléculaire observé: 100-110 kDa
• Numéro d’acquisition GenBank: BC052950
• Symbole du gène: GPRIN1
• Identification du gène (NCBI): 114787
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GPRIN1 (G protein-regulated inducer of neurite outgrowth 1), also named GRIN1, may be involved in neurite outgrowth. Western blotting analysis has confirmed its expression in mouse neuroblastoma and rat pheochromocytoma cells. And both GPRIN1 and GNAO1 are membrane-bound proteins that are enriched in the growth cones of neurites (PMID:10480904). It has 2 isoforms produced by alternative splicing. This antibody detects GRIN1 at bands around 110 kDa.

Publications

Species	Application	Title
mouse	WB,IHC,IF	Sci Signal; Dynamic interactions of the 5-HT6 receptor with protein partners control dendritic tree morphogenesis.; Authors - Camille N Pujol ; KD Validated
mouse	WB	J Biol Chem; DHHC5 interacts with PDZ domain 3 of post-synaptic density-95 (PSD-95) protein and plays a role in learning and memory.; Authors - Li Yi Y
human	WB	Exp Ther Med; Chronic ethanol exposure induces SK-N-SH cell apoptosis by increasing N-methyl-D-aspartic acid receptor expression and intracellular calcium.; Authors - Hongbo Wang

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