Anticorps Recombinant de lapin anti-GRP94

GRP94 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

CloneNo.

230502B7

N° de cat : 83147-5-RR

Synonymes

HSP90B1, 230502B7, 94 kDa glucose-regulated protein, ECGP, Endoplasmin

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Galerie de données de validation

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  WB analysis using 83147-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83147-5-RR (GRP94 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 83147-5-RR

  IP result of anti-GRP94 (IP:83147-5-RR, 4ug; Detection:83147-5-RR 1:5000) with HeLa cells lysate 1260 ug.

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  IHC staining of human lung cancer using 83147-5-RR

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 83147-5-RR (GRP94 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 83147-5-RR

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 83147-5-RR (GRP94 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 83147-5-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GRP94 antibody (83147-5-RR, Clone: 230502B7 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

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  IF Staining of HeLa using 83147-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GRP94 antibody (83147-5-RR, Clone: 230502B7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of HEK-293 using 83147-5-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using GRP94 antibody (83147-5-RR, Clone: 230502B7 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  FC experiment of A431 using 83147-5-RR

  1x10^6 A431 cells were intracellularly stained with 0.25 ug Grp94 Recombinant Antibody (83147-5-RR, Clone:230502B7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83147-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83147-5-RR against Human GRP94 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HepG2, cellules Jurkat, cellules LNCaP, tissu cérébral de porc
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de cancer du poumon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa, cellules HEK-293
• Résultats positifs en FC (Intra): cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83147-5-RR cible GRP94 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: GRP94 Protéine recombinante Ag1439
• Nom complet: heat shock protein 90kDa beta (Grp94), member 1
• Masse moléculaire calculée: 96 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC009195
• Symbole du gène: GRP94
• Identification du gène (NCBI): 7184
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Glucose-regulated protein 94 (GRP94), also called HSP90B1 and GP96, is an endoplasmic reticulum (ER)-resident member of the heat shock protein 90 (HSP90) family (PMID: 33802964; 24658275). Under ER stress conditions, GRP94 accelerates its function as a molecular chaperone. Client proteins of GRP94 include toll-like receptors (TLRs), glycoprotein (GP) IX subunit, insulin-like growth factors (IGFs), proinsulin, and integrins (PMID: 7913987; 32781621; 22079671). As well as being an ER chaperone, GRP94 can also participate in Calcium regulation and is also a regulator of innate and adaptive immunity (PMID: 33802964; 11584270).

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