- Phare
- Validé par KD/KO
Anticorps Polyclonal de lapin anti-GSDMD
GSDMD Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, rat, souris et plus (5)
Applications
WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Conjugaison
Non conjugué
N° de cat : 20770-1-AP
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en WB | cellules A431, cellules C6, cellules Jurkat, cellules NIH/3T3, tissu cérébral de rat, tissu hépatique de rat |
Résultats positifs en IP | cellules THP-1, |
Résultats positifs en IHC | human ovary cancer tissue, tissu pulmonaire de souris il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. |
Résultats positifs en IF/ICC | cellules HeLa, |
Résultats positifs en FC (Intra) | cellules Jurkat, |
Dilution recommandée
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunoprécipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochimie (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Applications publiées
KD/KO | See 7 publications below |
WB | See 296 publications below |
IHC | See 74 publications below |
IF | See 100 publications below |
IP | See 1 publications below |
Informations sur le produit
20770-1-AP cible GSDMD dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris
Réactivité | Humain, rat, souris |
Réactivité citée | rat, bovin, Humain, Lapin, poisson-zèbre, porc, souris, Hamster |
Hôte / Isotype | Lapin / IgG |
Clonalité | Polyclonal |
Type | Anticorps |
Immunogène | GSDMD Protéine recombinante Ag14757 |
Nom complet | gasdermin D |
Masse moléculaire calculée | 484 aa, 53 kDa |
Poids moléculaire observé | 50-53 kDa |
Numéro d’acquisition GenBank | BC008904 |
Symbole du gène | GSDMD |
Identification du gène (NCBI) | 79792 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par affinité contre l'antigène |
Tampon de stockage | PBS with 0.02% sodium azide and 50% glycerol |
Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
GSDMD is a 53 kDa cytosolic protein and a member of the gasdermin protein family, which features 6 members in humans (GSDMA, -B, -C, -D, -E and -F. GSDMD was identified as the executioner of inflammasome-associated pyroptosis. Caspase-1, -11, or -4 cleave GSDMD after an aspartate residue within a central linker domain (D275 in humans and D276 in mice) to generate a 31-kDa N-terminal GSDMD fragment (GSDMD-NT) and a 22-kDa C-terminal fragment (GSDMD-CT). In addition to the canonical 31/22 kDa cleavage products, a 43 kDa fragment has been observed under specific experimental or pathological conditions. The generation of the 43 kDa fragment was observed upon caspase-3 and -7 activation during apoptosis. This antibody can only detect full-length GSDMD.
Protocole
Product Specific Protocols | |
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WB protocol for GSDMD antibody 20770-1-AP | Download protocol |
IHC protocol for GSDMD antibody 20770-1-AP | Download protocol |
IF protocol for GSDMD antibody 20770-1-AP | Download protocol |
IP protocol for GSDMD antibody 20770-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Type III interferons induce pyroptosis in gut epithelial cells and impair mucosal repair | ||
Nature Inflammasome-activated gasdermin D causes pyroptosis by forming membrane pores.
| ||
ACS Nano Engineering Extracellular Vesicles Restore the Impaired Cellular Uptake and Attenuate Intervertebral Disc Degeneration. | ||
Acta Pharm Sin B Miltirone induces cell death in hepatocellular carcinoma cell through GSDME-dependent pyroptosis. | ||
Acta Pharm Sin B A high-throughput Gaussia luciferase reporter assay for screening potential gasdermin E activators against pancreatic cancer | ||
Cell Res Gasdermin D is an executor of pyroptosis and required for interleukin-1β secretion. |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Isha (Verified Customer) (03-02-2021) | Bands were so clear. Could not refrain myself from writing this review.
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