• Phare
  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-GSDMD

GSDMD Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (5)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications(376)

N° de cat : 20770-1-AP

Synonymes

FKSG10, gasdermin D, Gasdermin domain-containing protein 1, gasderminD, Gasdermin-D



Applications testées

Résultats positifs en WBcellules A431, cellules C6, cellules Jurkat, cellules NIH/3T3, tissu cérébral de rat, tissu hépatique de rat
Résultats positifs en IPcellules THP-1,
Résultats positifs en IHChuman ovary cancer tissue, tissu pulmonaire de souris
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
Résultats positifs en IF/ICCcellules HeLa,
Résultats positifs en FC (Intra)cellules Jurkat,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochimie (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

20770-1-AP cible GSDMD dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Réactivité citéerat, bovin, Humain, Lapin, poisson-zèbre, porc, souris, Hamster
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène GSDMD Protéine recombinante Ag14757
Nom complet gasdermin D
Masse moléculaire calculée 484 aa, 53 kDa
Poids moléculaire observé 50-53 kDa
Numéro d’acquisition GenBankBC008904
Symbole du gène GSDMD
Identification du gène (NCBI) 79792
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS with 0.02% sodium azide and 50% glycerol
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GSDMD is a 53 kDa cytosolic protein and a member of the gasdermin protein family, which features 6 members in humans (GSDMA, -B, -C, -D, -E and -F. GSDMD was identified as the executioner of inflammasome-associated pyroptosis. Caspase-1, -11, or -4 cleave GSDMD after an aspartate residue within a central linker domain (D275 in humans and D276 in mice) to generate a 31-kDa N-terminal GSDMD fragment (GSDMD-NT) and a 22-kDa C-terminal fragment (GSDMD-CT). In addition to the canonical 31/22 kDa cleavage products, a 43 kDa fragment has been observed under specific experimental or pathological conditions. The generation of the 43 kDa fragment was observed upon caspase-3 and -7 activation during apoptosis. This antibody can only detect full-length GSDMD.

Protocole

Product Specific Protocols
WB protocol for GSDMD antibody 20770-1-APDownload protocol
IHC protocol for GSDMD antibody 20770-1-APDownload protocol
IF protocol for GSDMD antibody 20770-1-APDownload protocol
IP protocol for GSDMD antibody 20770-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell

Type III interferons induce pyroptosis in gut epithelial cells and impair mucosal repair

Authors - Kautilya K Jena
humanWB

Nature

Inflammasome-activated gasdermin D causes pyroptosis by forming membrane pores.

Authors - Xing Liu
  • KD Validated
humanWB

ACS Nano

Engineering Extracellular Vesicles Restore the Impaired Cellular Uptake and Attenuate Intervertebral Disc Degeneration.

Authors - Zhiwei Liao
humanWB

Acta Pharm Sin B

Miltirone induces cell death in hepatocellular carcinoma cell through GSDME-dependent pyroptosis.

Authors - Xiaowei Zhang
humanWB

Acta Pharm Sin B

A high-throughput Gaussia luciferase reporter assay for screening potential gasdermin E activators against pancreatic cancer

Authors - Yang Liu
HumanWB

Cell Res

Gasdermin D is an executor of pyroptosis and required for interleukin-1β secretion.

Authors - Wan-Ting He

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Isha (Verified Customer) (03-02-2021)

Bands were so clear. Could not refrain myself from writing this review.

  • Applications: Western Blot, Immunohistochemistry
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mice kidney
{{ptg:RelatedPrimaryAntibodies}}