Anticorps Polyclonal de lapin anti-eRF3a/GSPT1

• Phare
• Validé par KD/KO

eRF3a/GSPT1 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

15

N° de cat : 10763-1-AP

Synonymes

551G9.2, eRF3a, ETF3A, G1 to S phase transition 1, GSPT1, GST1

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Galerie de données de validation

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  WB analysis using 10763-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10763-1-AP (eRF3a/GSPT1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10763-1-AP

  WB result of eRF3a/GSPT1 antibody (10763-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-eRF3a/GSPT1 transfected HepG2 cells.

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  WB analysis using 10763-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10763-1-AP (eRF3a/GSPT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 10763-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10763-1-AP (eRF3a/GSPT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 10763-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10763-1-AP (eRF3a/GSPT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 10763-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10763-1-AP (eRF3a/GSPT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 10763-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10763-1-AP (eRF3a/GSPT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 10763-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10763-1-AP (eRF3a/GSPT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10763-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10763-1-AP (eRF3a/GSPT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 10763-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using eRF3a/GSPT1 antibody (10763-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules Caco-2, cellules HCT 116, cellules HeLa, cellules HepG2, cellules MOLT-4
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu cérébral de souris, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 13 publications below
• IHC: See 2 publications below
• IF: See 4 publications below

Informations sur le produit

10763-1-AP cible eRF3a/GSPT1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: eRF3a/GSPT1 Protéine recombinante Ag1184
• Nom complet: G1 to S phase transition 1
• Masse moléculaire calculée: 68 aa, 4 kDa
• Poids moléculaire observé: 80-85 kDa
• Numéro d’acquisition GenBank: BC009503
• Symbole du gène: GSPT1
• Identification du gène (NCBI): 2935
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The eukaryotic Release Factor 3 (eRF3) is a GTPase that associates with eRF1 in a complex that mediates translation termination. Eukaryotic release factor 3 (eRF3) has many functions in eukaryotic cells, such as controlling the regulation of the cell cycle at the G1 to S phase transition, and regulating protein synthesis as a GTP dependent stimulator of eRF1 in translation termination. It was also reported to play a key role as an initiator of the mRNA degradation machinery in the recycling of ribosomes in successive cycles of translation,and probably also in transcription regulation. eRF3a, also known as GSPT1, is one subunit of eRF3(PMID:15917414,12923185). It involves in translation termination in response to the termination codons UAA, UAG and UGA and stimulates the activity of ERF1. eRF3a/GSPT1 exists some isoforms with MV 69 kDa and 56 kDa. Identification of a processed form of eRF3a/GSPT1 as a BIR3-binding protein-Using a GST-BIR3 fusion protein as an affinity reagent to purify new IAP binding proteins from extracts of human cells and mouse tissues, we previously isolated 3 proteins of molecular weights 23, 38 and 80 kDa. 80 kDa band confirmed that it is a processed form of the human GSPT1/eRF3a protein, lacking the first 69 residues(PMID: 12865429).

Publications

Species	Application	Title
human	WB	Nature; A novel cereblon modulator recruits GSPT1 to the CRL4(CRBN) ubiquitin ligase.; Authors - Mary E Matyskiela ; KD Validated
human	IF	Mol Cell; Spatiotemporal Proteomic Analysis of Stress Granule Disassembly Using APEX Reveals Regulation by SUMOylation and Links to ALS Pathogenesis.; Authors - Hagai Marmor-Kollet
human, mouse	WB	Mol Cell; Mammalian hyperplastic discs homolog EDD regulates miRNA-mediated gene silencing.; Authors - Hong Su
human	WB,IF	Cardiovasc Res; Readthrough of nonsense mutation W822X in the SCN5A gene can effectively restore expression of cardiac Na+ channels.; Authors - Teng Siyong S ; KD Validated
human	WB	J Biol Chem; A Novel Rac1-GSPT1 Signaling Pathway Controls Astrogliosis Following Central Nervous System Injury.; Authors - Taiji Ishii
human	WB, IF	Mol Cancer; Nicotine-mediated invasion and migration of non-small cell lung carcinoma cells by modulating STMN3 and GSPT1 genes in an ID1-dependent manner.; Authors - Sajitha Nair