Anticorps Polyclonal de lapin anti-GSS

• Phare
• Validé par KD/KO

GSS Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

canin, Humain, porc, rat, souris, Hamster et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(24)

Avis (1)

N° de cat : 15712-1-AP

Synonymes

GSHS, GSH synthetase, GSH S, glutathione synthetase, Glutathione synthase

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Galerie de données de validation

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  WB analysis using 15712-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15712-1-AP (GSS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 15712-1-AP

  WB result of GSS antibody (15712-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSS transfected HEK-293 cells.

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  IHC staining of human kidney using 15712-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15712-1-AP (GSS Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 15712-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15712-1-AP (GSS Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of MDCK using 15712-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MDCK cells using 15712-1-AP (GSS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules CHO, cellules HEK-293, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IHC: tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MDCK

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 22 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Informations sur le produit

15712-1-AP cible GSS dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons canin, Humain, porc, rat, souris, Hamster

• Réactivité: canin, Humain, porc, rat, souris, Hamster
• Réactivité citée: rat, Humain, porc, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GSS Protéine recombinante Ag8338
• Nom complet: glutathione synthetase
• Masse moléculaire calculée: 474 aa, 52 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC007927
• Symbole du gène: GSS
• Identification du gène (NCBI): 2937
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse,human	WB,IHC,IF	ACS Nano; Engineered Extracellular Vesicle-Delivered CRISPR/Cas9 for Radiotherapy Sensitization of Glioblastoma; Authors - Xiao Liu
human	WB	Adv Sci (Weinh); Paracrine Orchestration of Tumor Microenvironment Remodeling Induced by GLO1 Potentiates Lymph Node Metastasis in Breast Cancer; Authors - Jindong Xie
human	WB	Oxid Med Cell Longev; IMCA Induces Ferroptosis Mediated by SLC7A11 through the AMPK/mTOR Pathway in Colorectal Cancer.; Authors - Lei Zhang
mouse	WB	Redox Biol; γ-glutamylcysteine exhibits anti-inflammatory effects by increasing cellular glutathione level.; Authors - Yang Yang
human	WB	Pharmacol Res; Phyto-sesquiterpene lactones DET and DETD-35 induce ferroptosis in vemurafenib sensitive and resistant melanoma via GPX4 inhibition and metabolic reprogramming.; Authors - Meng-Ting Chang
mouse	WB	FASEB J; Taurine treatment prevents derangement of the hepatic γ-glutamyl cycle and methylglyoxal metabolism in a mouse model of classical homocystinuria: regulatory crosstalk between thiol and sulfinic acid metabolism.; Authors - Kenneth N Maclean

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hua (Verified Customer) (08-05-2021)

This is the second tube I purchased from ProteinTech. I got my first tube of this antibody in 2016 and it worked well. However, it suddenly stopped working last year. There is no signal at all on the membrane. Thus, I purchased the second tube in May 2021 and I just used it on my experiments as my previous record showing it is working well in WB. Unfortunately, I couldn't get any signal at all. I ran two tests with this antibody and it shows it stopping work with mouse liver.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human liver, mouse liver, mouse brain and mouse kidney.

GSS Antibody Western Blot validation (1:1000 dilution) in Human liver, mouse liver, mouse brain and mouse kidney. (Cat no:15712-1-AP)

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