- Phare
- Validé par KD/KO
Anticorps Polyclonal de lapin anti-GSS
GSS Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
canin, Humain, porc, rat, souris, Hamster et plus (1)
Applications
WB, IHC, IF/ICC, ELISA
Conjugaison
Non conjugué
N° de cat : 15712-1-AP
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | cellules CHO, cellules HEK-293, tissu hépatique de rat, tissu hépatique de souris |
| Résultats positifs en IHC | tissu rénal humain il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. |
| Résultats positifs en IF/ICC | cellules MDCK |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochimie (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| WB | See 22 publications below |
| IHC | See 2 publications below |
| IF | See 3 publications below |
Informations sur le produit
15712-1-AP cible GSS dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons canin, Humain, porc, rat, souris, Hamster
| Réactivité | canin, Humain, porc, rat, souris, Hamster |
| Réactivité citée | rat, Humain, porc, poulet, souris |
| Hôte / Isotype | Lapin / IgG |
| Clonalité | Polyclonal |
| Type | Anticorps |
| Immunogène | GSS Protéine recombinante Ag8338 |
| Nom complet | glutathione synthetase |
| Masse moléculaire calculée | 474 aa, 52 kDa |
| Poids moléculaire observé | 52 kDa |
| Numéro d’acquisition GenBank | BC007927 |
| Symbole du gène | GSS |
| Identification du gène (NCBI) | 2937 |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par affinité contre l'antigène |
| Tampon de stockage | PBS with 0.02% sodium azide and 50% glycerol |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for GSS antibody 15712-1-AP | Download protocol |
| IHC protocol for GSS antibody 15712-1-AP | Download protocol |
| IF protocol for GSS antibody 15712-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
ACS Nano Engineered Extracellular Vesicle-Delivered CRISPR/Cas9 for Radiotherapy Sensitization of Glioblastoma | ||
Adv Sci (Weinh) Paracrine Orchestration of Tumor Microenvironment Remodeling Induced by GLO1 Potentiates Lymph Node Metastasis in Breast Cancer | ||
Oxid Med Cell Longev IMCA Induces Ferroptosis Mediated by SLC7A11 through the AMPK/mTOR Pathway in Colorectal Cancer. | ||
Redox Biol γ-glutamylcysteine exhibits anti-inflammatory effects by increasing cellular glutathione level. | ||
Pharmacol Res Phyto-sesquiterpene lactones DET and DETD-35 induce ferroptosis in vemurafenib sensitive and resistant melanoma via GPX4 inhibition and metabolic reprogramming. | ||
FASEB J Taurine treatment prevents derangement of the hepatic γ-glutamyl cycle and methylglyoxal metabolism in a mouse model of classical homocystinuria: regulatory crosstalk between thiol and sulfinic acid metabolism. |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Hua (Verified Customer) (08-05-2021) | This is the second tube I purchased from ProteinTech. I got my first tube of this antibody in 2016 and it worked well. However, it suddenly stopped working last year. There is no signal at all on the membrane. Thus, I purchased the second tube in May 2021 and I just used it on my experiments as my previous record showing it is working well in WB. Unfortunately, I couldn't get any signal at all. I ran two tests with this antibody and it shows it stopping work with mouse liver.
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