Anticorps Monoclonal anti-GST Tag

GST Tag Monoclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Protéine recombinante, schistosoma japonicum et plus (1)

Applications

WB, IP, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

91

CloneNo.

3G12B10

Avis (3)

N° de cat : 66001-2-Ig

Synonymes

GST, GST Tag

---

• Galerie de données de validation
• Proteintech vs. Other Vendors

• 

  WB analysis of Recombinant protein using 66001-2-Ig

  GST tagged recombinant protein (25ng/lane) was subjected to SDS-PAGE followed by western blot with anti-GST tag monoclonal antibody 66001-2-Ig at various dilutions.

• 

  WB analysis of Recombinant protein using 66001-2-Ig

  GST protein (25 ng), pGEX6P-1 vector expressed in E. coli, were subjected to SDS PAGE followed by western blot with 66001-2-Ig (GST Tag antibody, 1:200,000) incubated at room temperature for 1.5 hours.

• 

  IP experiment of Recombinant protein using 66001-2-Ig

  IP result of anti-GST Tag(IP:66001-2-Ig, 5ug; Detection:66001-2-Ig 1:10000) with Total E. coli lysate (with pGEX4T fused plasmid) 1360 ug.

"GST Tag Antibodies" Comparison

View side-by-side comparison of GST Tag antibodies from other vendors to find the one that best suits your research needs.

Compare now

---

Applications testées

• Résultats positifs en WB: Protéine recombinante,
• Résultats positifs en IP: Lysat d'E. coli

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 75 publications below
• IF: See 4 publications below
• IP: See 15 publications below
• CoIP: See 3 publications below

Informations sur le produit

66001-2-Ig cible GST Tag dans les applications de WB, IP, IF, CoIP, ELISA et montre une réactivité avec des échantillons Protéine recombinante, schistosoma japonicum

• Réactivité: Protéine recombinante, schistosoma japonicum
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: GST Tag
• Masse moléculaire calculée: 26 kDa
• Numéro d’acquisition GenBank: FN315687
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. GST (Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. Antibodies to GST are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody recognizes the GST-tag fused to either the N- or C-terminus of targeted proteins.

Publications

Species	Application	Title
human	WB	Nat Cancer; METTL16 antagonizes MRE11-mediated DNA end resection and confers synthetic lethality to PARP inhibition in pancreatic ductal adenocarcinoma; Authors - Xiangyu Zeng
	WB	Nat Cell Biol; Phagolysosome resolution requires contacts with the endoplasmic reticulum and phosphatidylinositol-4-phosphate signalling.; Authors - Roni Levin-Konigsberg
	WB	Sci Bull (Beijing); Targeted clearance of mitochondria by an autophagy-tethering compound (ATTEC) and its potential therapeutic effects; Authors - Shuixia Tan
	WB	Nat Commun; Fungal secondary metabolism is governed by an RNA-binding protein CsdA/RsdA complex; Authors - Zili Song
	WB	Nat Commun; N-terminal α-amino SUMOylation of cofilin-1 is critical for its regulation of actin depolymerization; Authors - Weiji Weng
	WB,IP	Adv Sci (Weinh); Elevated Nuclear PHGDH Synergistically Functions with cMyc to Reshape the Immune Microenvironment of Liver Cancer; Authors - Hongwen Zhu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Lana (Verified Customer) (05-27-2022)

SDS-PAGE: 4-12% Bis-Tris gradient gel. Transfer: Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4C. Blocking: SEA Block Blocking Buffer 1h, room T. Primary Ab: O/N incubation at 4C, 1:1000. Secondary Ab: IRDye 680LT Goat anti-Mouse, 1:15000. Lines of WB image: 1 – protein ladder, 2 and 3 – GST protein different amounts.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Lysate of Sepharose beads after GST-pull down

GST Tag Antibody Western Blot validation (1:5000 dilution) in Lysate of Sepharose beads after GST-pull down (Cat no:66001-2-Ig)

FH

Susanne (Verified Customer) (11-18-2021)

Clear Signal, no background on E.coli lysate with expressed GST blotted on PVDF membrane

• Applications: Western Blot, Cell culture
• Primary Antibody Dilution: 1:30000
• Cell Tissue Type: E.coli lysate

FH

Aurelie (Verified Customer) (12-11-2018)

• Applications: Western Blot, Immunoprecipitation,
• Primary Antibody Dilution: 1µl for IP and 1/5000 for WB
• Cell Tissue Type: in vitro

GST Tag Antibody Western Blot,Immunoprecipitation, validation (1µl for IP and 1/5000 for WB dilution) in in vitro (Cat no:66001-2-Ig)