GST Tag Polyclonal antibody

GST Tag Polyclonal Antibody for FC, WB,ELISA

Host / Isotype

Rabbit / IgG


recombinant protein





Cat no : 10000-0-AP


Glutathione S-Transferase, GST, gst tag

"GST Tag Antibodies" Comparison

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  • GST Tag Antibody WB recombinant protein  10000-0-AP

    WB analysis of recombinant protein using 10000-0-AP

    Western blot of GST-tagged fusion protein with anti-GST-tag (10000-0-AP) at various dilutions.

  • FC experiment of HeLa using 10000-0-AP

    FC experiment of HeLa using 10000-0-AP

    1X10^6 HeLa cells were stained with 0.20 ug PSMD9 antibody (26922-1-AP, red) and 0.20 ug Rabbit IgG control (10000-0-AP, blue). Fixed with 90% MeOH.

Tested Applications

Positive WB detected inRecombinant protein
Positive FC detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Product Information

10000-0-AP targets GST Tag in WB, IP, IF, FC, ChIP, ELISA applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant Protein
Full Name GST Tag
Calculated molecular weight 26 kDa
GenBank accession numberFN315687
Gene symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GST(Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Antibodies to GST are useful for checking protein expression both in plaques and on western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody can be used to detect the GST tagged protein. This antibody can be used as Rabbit IgG control in FC.



Cell Death Differ

STING signaling remodels the tumor microenvironment by antagonizing myeloid-derived suppressor cell expansion.

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Nat Commun

Tyrosine phosphorylation activates 6-phosphogluconate dehydrogenase and promotes tumor growth and radiation resistance.

Authors - Ruilong Liu

Cancer Lett

PMID: 30872078

Authors - Jie Yang


Neuroprotective Effect of Dioscin on the Aging Brain.

Authors - Yan Qi

Int J Cancer

Heparanase promotes glioma progression via enhancing CD24 expression.

Authors - Uri Barash


Cortisol Excess-Mediated Mitochondrial Damage Induced Hippocampal Neuronal Apoptosis in Mice Following Cold Exposure.

Authors - Bin Xu


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Daniel (Verified Customer) (06-10-2022)

We use this anti-GST antibody for GST fusion protein-lipid interactions. We found this antibody gives much less background than other antibodies.

  • Applications: Other
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: not applicable
GST Tag Antibody Other validation (1:1000 dilution) in not applicable (Cat no:10000-0-AP)

Lana (Verified Customer) (04-06-2022)

SDS-PAGE: 4-12% Bis-Tris gradient gel. Transfer: Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4C. Blocking: SEA Block Blocking Buffer 1h, room T. Primary Ab: O/N incubation at 4C, 1:1000. Secondary Ab: IRDye 680LT Goat anti-Rabbit, 1:15000. Lines of WB image: 1 – protein ladder, 2 – mitochondria fraction lysate, 3 – GST protein, 4 – GST fusion protein

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Lysate of Sepharose beads after GST-pull down
GST Tag Antibody Western Blot validation (1:1000 dilution) in Lysate of Sepharose beads after GST-pull down (Cat no:10000-0-AP)

Shihab (Verified Customer) (10-28-2021)

Tried this antibody for a control experiment and worked nicely. Will be continuing to try this in immunofluorescence

  • Applications: Western Blot
  • Cell Tissue Type: Transfected HEK293

Amie (Verified Customer) (07-27-2021)

I compared this antibody directly to the proteintech mouse monoclonal (66001-2-Ig). In the provided image, the first 4 lanes of each gel are identical. The rabbit antibody seems to be slightly better (less background) than the mouse.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: recombinant GST-tagged proteins expressed in yeast
GST Tag Antibody Western Blot validation (1:5000 dilution) in recombinant GST-tagged proteins expressed in yeast (Cat no:10000-0-AP)

Yan (Verified Customer) (02-20-2021)

It worked very well when I performed western blot after immunoprecipitation.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: mouse adult heart