|Positive WB detected in||Recombinant protein|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Sample-dependent, check data in validation data gallery|
10000-0-AP targets GST Tag in WB, IP, IF, FC, ChIP, ELISA applications and shows reactivity with recombinant protein samples.
|Tested Reactivity||recombinant protein|
|Host / Isotype||Rabbit / IgG|
|Full Name||GST Tag|
|Calculated molecular weight||26 kDa|
|GenBank accession number||FN315687|
|Gene ID (NCBI)|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
GST(Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Antibodies to GST are useful for checking protein expression both in plaques and on western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody can be used to detect the GST tagged protein. This antibody can be used as Rabbit IgG control in FC.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Daniel (Verified Customer) (06-10-2022)
We use this anti-GST antibody for GST fusion protein-lipid interactions. We found this antibody gives much less background than other antibodies.
Lana (Verified Customer) (04-06-2022)
SDS-PAGE: 4-12% Bis-Tris gradient gel. Transfer: Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4C. Blocking: SEA Block Blocking Buffer 1h, room T. Primary Ab: O/N incubation at 4C, 1:1000. Secondary Ab: IRDye 680LT Goat anti-Rabbit, 1:15000. Lines of WB image: 1 – protein ladder, 2 – mitochondria fraction lysate, 3 – GST protein, 4 – GST fusion protein
Shihab (Verified Customer) (10-28-2021)
Tried this antibody for a control experiment and worked nicely. Will be continuing to try this in immunofluorescence
Amie (Verified Customer) (07-27-2021)
I compared this antibody directly to the proteintech mouse monoclonal (66001-2-Ig). In the provided image, the first 4 lanes of each gel are identical. The rabbit antibody seems to be slightly better (less background) than the mouse.
Yan (Verified Customer) (02-20-2021)
It worked very well when I performed western blot after immunoprecipitation.