GST Tag Polyclonal antibody

• Validation Data Gallery
• Proteintech vs. Other Vendors

• 

  WB analysis of recombinant protein using 10000-0-AP

  Western blot of GST-tagged fusion protein with anti-GST-tag (10000-0-AP) at various dilutions.

• 

  WB analysis using 10000-0-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10000-0-AP (GST Tag antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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GST Tag Polyclonal Antibody for WB, ELISA

Cat No. 10000-0-AP

Publications(241)

Reviews (5)

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein and More (1)

Applications

WB, IF, IP, CoIP, ChIP, ELISA

Synonyms

Glutathione S-Transferase, GST

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Tested Applications

• Positive WB detected in: Recombinant protein

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 162 publications below
• IF: See 3 publications below
• IP: See 29 publications below
• ELISA: See 1 publications below
• CoIP: See 5 publications below
• ChIP: See 2 publications below

Product Information

10000-0-AP targets GST Tag in WB, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with recombinant protein samples.

• Tested Reactivity: recombinant protein
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: GST Tag
• Calculated Molecular Weight: 26 kDa
• GenBank Accession Number: FN315687
• RRID: AB_11042316
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GST(Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Antibodies to GST are useful for checking protein expression both in plaques and on western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody can be used to detect the GST tagged protein. This antibody can be used as Rabbit IgG control in FC.

Publications

Species	Application	Title
	IP	Cell; The WDR11 complex is a receptor for acidic-cluster-containing cargo proteins; Authors - Huaqing Deng
	WB,IP	Signal Transduct Target Ther; SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis; Authors - Saisai Guo
	WB	Gastroenterology; PTEN deficiency facilitates exosome secretion and metastasis in cholangiocarcinoma by impairing TFEB-mediated lysosome biogenesis; Authors - Tian-Yi Jiang
		Ann Rheum Dis; Autoantibodies identify primary Sjögren's syndrome in patients lacking serum IgG specific for Ro/SS-A and La/SS-B; Authors - Sherri Longobardi
	WB	Immunity; Targeting 7-Dehydrocholesterol Reductase Integrates Cholesterol Metabolism and IRF3 Activation to Eliminate Infection.; Authors - Jun Xiao
	WB	Nat Cell Biol; O-GlcNAcylation determines the translational regulation and phase separation of YTHDF proteins; Authors - Yulin Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Daniel (Verified Customer) (06-10-2022)

We use this anti-GST antibody for GST fusion protein-lipid interactions. We found this antibody gives much less background than other antibodies.

• Applications: Other
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: not applicable

GST Tag Antibody Other validation (1:1000 dilution) in not applicable (Cat no:10000-0-AP)

FH

Lana (Verified Customer) (04-06-2022)

SDS-PAGE: 4-12% Bis-Tris gradient gel. Transfer: Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4C. Blocking: SEA Block Blocking Buffer 1h, room T. Primary Ab: O/N incubation at 4C, 1:1000. Secondary Ab: IRDye 680LT Goat anti-Rabbit, 1:15000. Lines of WB image: 1 – protein ladder, 2 – mitochondria fraction lysate, 3 – GST protein, 4 – GST fusion protein

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Lysate of Sepharose beads after GST-pull down

GST Tag Antibody Western Blot validation (1:1000 dilution) in Lysate of Sepharose beads after GST-pull down (Cat no:10000-0-AP)

FH

Shihab (Verified Customer) (10-28-2021)

Tried this antibody for a control experiment and worked nicely. Will be continuing to try this in immunofluorescence

• Applications: Western Blot
• Cell Tissue Type: Transfected HEK293

FH

Amie (Verified Customer) (07-27-2021)

I compared this antibody directly to the proteintech mouse monoclonal (66001-2-Ig). In the provided image, the first 4 lanes of each gel are identical. The rabbit antibody seems to be slightly better (less background) than the mouse.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: recombinant GST-tagged proteins expressed in yeast

GST Tag Antibody Western Blot validation (1:5000 dilution) in recombinant GST-tagged proteins expressed in yeast (Cat no:10000-0-AP)

FH

Yan (Verified Customer) (02-20-2021)

It worked very well when I performed western blot after immunoprecipitation.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse adult heart