GST Tag Polyclonal antibody

GST Tag Polyclonal Antibody for FC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein

Applications

WB, IP, FC, ELISA

Conjugate

Unconjugated

Publications

59

Cat no : 10000-0-AP

Synonyms

Glutathione S-Transferase, GST, gst tag

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Validation Data Gallery

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  FC experiment of HeLa using 10000-0-AP

  1X10^6 HeLa cells were stained with 0.20 ug PSMD9 antibody (26922-1-AP, red) and 0.20 ug Rabbit IgG control (10000-0-AP, blue). Fixed with 90% MeOH .

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  WB analysis of recombinant protein protein using 10000-0-AP

  Western blot of GST-tagged fusion protein with anti-GST-tag (10000-0-AP) at various dilutions.

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Tested Applications

• Positive WB detected in: Recombinant protein
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 20 publications below
• IP: See 1 publications below

Product Information

10000-0-AP targets GST Tag in WB, IP, FC, ELISA applications and shows reactivity with recombinant protein samples.

• Tested Reactivity: recombinant protein
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: GST Tag
• Calculated molecular weight: 26 kDa
• GenBank accession number: FN315687
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

GST(Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Antibodies to GST are useful for checking protein expression both in plaques and on western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody can be used to detect the GST tagged protein. This antibody can be used as Rabbit IgG control in FC.

Publications

Species	Application	Title
		Cell Death Differ; STING signaling remodels the tumor microenvironment by antagonizing myeloid-derived suppressor cell expansion.; Authors - Chuan-Xia Zhang
		Nat Commun; Tyrosine phosphorylation activates 6-phosphogluconate dehydrogenase and promotes tumor growth and radiation resistance.; Authors - Ruilong Liu
		Cancer Lett; PMID: 30872078; Authors - Jie Yang
		Molecules; Neuroprotective Effect of Dioscin on the Aging Brain.; Authors - Yan Qi
		Int J Cancer; Heparanase promotes glioma progression via enhancing CD24 expression.; Authors - Uri Barash
		Cells; Cortisol Excess-Mediated Mitochondrial Damage Induced Hippocampal Neuronal Apoptosis in Mice Following Cold Exposure.; Authors - Bin Xu