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GST Tag Antibody 36 Publications

Rabbit Polyclonal| Catalog number: 10000-0-AP

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Con: 77 μg/150 μl

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Tested Applications

Applications:
WB, IP, FC, ELISA
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Images See all

GST Tag Antibody FC HeLa cells 10000-0-AP

1X10^6 HeLa cells were stained with 0.20 ug PSMD9 antibody (26922-1-AP, red) and 0.20 ug Rabbit IgG control (10000-0-AP, blue). Fixed with 90% MeOH

GST Tag Antibody WB recombinant protein protein 10000-0-AP

Western blot of GST-tagged fusion protein with anti-GST-tag (10000-0-AP) at various dilutions.

Species specificity:
recombinant protein, human
Positive WB detected in:
Recombinant protein
Positive FC detected in:
HeLa cells
Recommended dilution:
WB : 1:1000-1:4000

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Recombinant Protein
Full name:
GST Tag
Calculated molecular weight:
26 kDa
Observed molecular weight:
GenBank accession number:
FN315687
Gene ID (NCBI):
Gene symbol
Synonyms
Glutathione S-Transferase, GST, gst tag

Background

GST(Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Antibodies to GST are useful for checking protein expression both in plaques and on western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody can be used to detect the GST tagged protein. This antibody can be used as Rabbit IgG control in FC.


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