GST Tag Polyclonal antibody

GST Tag Polyclonal Antibody for FC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein

Applications

WB, IP, FC, ELISA

Conjugate

Unconjugated

Cat no : 10000-0-AP

Synonyms

Glutathione S-Transferase, GST, gst tag



Tested Applications

Positive WB detected inRecombinant protein
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Product Information

10000-0-AP targets GST Tag in WB, IP, FC, ELISA applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant Protein
Full Name GST Tag
Calculated molecular weight 26 kDa
GenBank accession numberFN315687
Gene symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GST(Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Antibodies to GST are useful for checking protein expression both in plaques and on western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody can be used to detect the GST tagged protein. This antibody can be used as Rabbit IgG control in FC.

Publications

SpeciesApplicationTitle

Cell Death Differ

STING signaling remodels the tumor microenvironment by antagonizing myeloid-derived suppressor cell expansion.

Authors - Chuan-Xia Zhang

Nat Commun

Tyrosine phosphorylation activates 6-phosphogluconate dehydrogenase and promotes tumor growth and radiation resistance.

Authors - Ruilong Liu

Cancer Lett

PMID: 30872078

Authors - Jie Yang

Molecules

Neuroprotective Effect of Dioscin on the Aging Brain.

Authors - Yan Qi

Int J Cancer

Heparanase promotes glioma progression via enhancing CD24 expression.

Authors - Uri Barash

Cells

Cortisol Excess-Mediated Mitochondrial Damage Induced Hippocampal Neuronal Apoptosis in Mice Following Cold Exposure.

Authors - Bin Xu