Anticorps Recombinant de lapin anti-GST Tag

GST Tag Recombinant Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Protéine recombinante et plus (1)

Applications

WB, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(4)

CloneNo.

9B24

N° de cat : 80006-1-RR

Synonymes

GST, Glutathione S-Transferase

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Galerie de données de validation

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  WB analysis using 80006-1-RR

  GST tagged protein, target band 42 kDa and degraded band 32 kDa, and other negative samples were subjected to SDS PAGE followed by western blot with 80006-1-RR (GST Tag antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of Recombinant protein using 80006-1-RR

  IP result of anti-GST Tag (IP:80006-1-RR, 4ug; Detection:80006-1-RR 1:5000) with Recombinant protein lysate 1200 ug.

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Applications testées

• Résultats positifs en WB: Protéine recombinante
• Résultats positifs en IP: Protéine recombinante,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

80006-1-RR cible GST Tag dans les applications de WB, IP, CoIP, ELISA et montre une réactivité avec des échantillons Protéine recombinante

• Réactivité: Protéine recombinante
• Réactivité citée: rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: GST Tag Protéine recombinante Ag0040
• Nom complet: GST Tag
• Masse moléculaire calculée: 26 kDa
• Numéro d’acquisition GenBank: FN315687
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GST (Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. Antibodies to GST are useful for checking protein expression both in plaques and on western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody is a Rabbit recombinant antibody to GST tag.

Publications

Species	Application	Title
rat	WB	Cell Rep; The STRIPAK complex is required for radial sorting and laminin receptor expression in Schwann cells; Authors - Michael R Weaver
		Acta Pharmacol Sin; Gracillin suppresses cancer progression through inducing Merlin/LATS protein-protein interaction and activating Hippo signaling pathway; Authors - Jin-Xuan Su
	WB	bioRxiv; The STRIPAK complex is required for radial sorting and laminin receptor expression in Schwann cells; Authors - Michael R Weaver
	CoIP	Vet Microbiol; PEDV N protein capture protein translation element PABPC1 and eIF4F to promote viral replication; Authors - Huanjie Zhai

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