Anticorps Polyclonal de lapin anti-GSTA1

• Phare
• Validé par KD/KO

GSTA1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

14

N° de cat : 14475-1-AP

Synonymes

Glutathione S transferase A1, GST class alpha member 1, GST epsilon, GST HA subunit 1, GST2, GSTA1, GSTA1 1, GTH1

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Galerie de données de validation

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  WB analysis using 14475-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14475-1-AP (GSTA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14475-1-AP

  WB result of GSTA1 antibody (14475-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSTA1 transfected HepG2 cells.

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  WB analysis of human kidney using 14475-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 14475-1-AP (GSTA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 14475-1-AP

  IP Result of anti-GSTA1 (IP:14475-1-AP, 3ug; Detection:14475-1-AP 1:500) with mouse liver tissue lysate 4000ug.

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  IHC staining of human lung cancer using 14475-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14475-1-AP (GSTA1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 14475-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14475-1-AP (GSTA1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 14475-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14475-1-AP (GSTA1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 14475-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 14475-1-AP (GSTA1 Antibody) at dilution of 1:50.

• 

  IHC staining of human kidney using 14475-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 14475-1-AP (GSTA1 Antibody) at dilution of 1:50.

• 

  IHC staining of human colon cancer using 14475-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14475-1-AP (GSTA1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 14475-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 14475-1-AP (GSTA1 Antibody) at dilution of 1:50.

• 

  IF Staining of HepG2 using 14475-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using GSTA1 antibody (14475-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, cellules HepG2, tissu hépatique de rat, tissu hépatique de souris, tissu rénal de rat, tissu rénal humain
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer du côlon humain, tissu hépatique humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 14 publications below
• IHC: See 1 publications below

Informations sur le produit

14475-1-AP cible GSTA1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GSTA1 Protéine recombinante Ag5849
• Nom complet: glutathione S-transferase alpha 1
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 26 kDa
• Numéro d’acquisition GenBank: BC053578
• Symbole du gène: GSTA1
• Identification du gène (NCBI): 2938
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GSTA1 encodes one of the GST alpha isozymes, belongs to GST family, which is mainly expressed in the liver. GST can improve the detoxification of organism by enzymatic and non-enzymatic methods, and has anti-mutagenic and anti-tumor effects. Studies have shown that overexpression of GSTA1 can promote the development of lung cancer cells, so the expression level of GSTA1 can be used to predict the degree of malignancy of lung cancer tissues. To some extent, GSTA1 is regarded as a potential lung cancer candidate targets.

Publications

Species	Application	Title
mouse	WB	Nat Commun; Pregnane X receptor agonist nomilin extends lifespan and healthspan in preclinical models through detoxification functions; Authors - Shengjie Fan
mouse	WB	Cell Death Dis; Poly(ADP-ribosyl)ated PXR is a critical regulator of acetaminophen-induced hepatotoxicity.; Authors - Cheng Wang
mouse	WB	J Gerontol A Biol Sci Med Sci; PMID: 24285747; Authors - Rojanathammanee Lalida L
mouse	WB	Age (Dordr); PMID: 25001375; Authors - Lalida Rojanathammanee
rat	WB	Food Chem Toxicol; l-Arginine induces antioxidant response to prevent oxidative stress via stimulation of glutathione synthesis and activation of Nrf2 pathway.; Authors - Mingcai Liang
rat	WB	PLoS One; Heat-Not-Burn cigarette induces oxidative stress response in primary rat alveolar epithelial cells.; Authors - Yoko Ito