Anticorps Polyclonal de lapin anti-GSTO1

• Phare
• Validé par KD/KO

GSTO1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

11

N° de cat : 15124-1-AP

Synonymes

GSTO 1, GSTO1, GSTTLp28, P28

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Galerie de données de validation

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  WB analysis using 15124-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15124-1-AP (GSTO1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 15124-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 15124-1-AP (GSTO1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 15124-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15124-1-AP (GSTO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 15124-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 15124-1-AP (GSTO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 15124-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 15124-1-AP (GSTO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 15124-1-AP

  IP Result of anti-GSTO1 (IP:15124-1-AP, 4ug; Detection:15124-1-AP 1:500) with Jurkat cells lysate 4000ug.

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  IHC staining of human pancreas cancer using 15124-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15124-1-AP (GSTO1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 15124-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15124-1-AP (GSTO1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of PC-3 using 15124-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using GSTO1 antibody (15124-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, cellules PC-3, cellules U-937, tissu cardiaque humain, tissu cérébral de rat, tissu hépatique de souris
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IHC: tissu de cancer du pancréas humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules PC-3,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IF: See 2 publications below

Informations sur le produit

15124-1-AP cible GSTO1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GSTO1 Protéine recombinante Ag7199
• Nom complet: glutathione S-transferase omega 1
• Masse moléculaire calculée: 28 kDa
• Poids moléculaire observé: 27-30 kDa
• Numéro d’acquisition GenBank: BC000127
• Symbole du gène: GSTO1
• Identification du gène (NCBI): 9446
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Glutathione-S-transferase omega 1 (GSTO1) belongs to a new subfamily of GSTs and is the rate limiting enzyme for biotransformation of inorganic arsenic, environmental carcinogen. Expression of GSTO1-1 was abundant in a wide range of normal tissues, particularly liver, macrophages, glial cells, and endocrine cells. Human GSTO1 assembles as a dimer. This antibody detects both monomer (27-30 kDa) and dimer forms of GSTO1 (54-64 kDa). (PMID: 21495794)

Publications

Species	Application	Title
human	WB	Mol Cell; SMURF2 predisposes cancer cell toward ferroptosis in GPX4-independent manners by promoting GSTP1 degradation; Authors - Wenxin Zhang
mouse	WB,IF	J Exp Med; ASC deglutathionylation is a checkpoint for NLRP3 inflammasome activation.; Authors - Shuhang Li ; KO Validated
pig	WB	Food Chem; TMT-based quantitative proteomic analysis of porcine muscle associated with postmortem meat quality.; Authors - Xinhua Hou
		Biochem J; Selenophosphate Synthetase 1 is an Essential Protein with Roles in Regulation of Redox Homeostasis in Mammals.; Authors - Ryuta Tobe
mouse	WB	Toxicol Appl Pharmacol; PMID: 24998975; Authors - Jing-Jing Chuang
human	WB	J Proteomics; Proteomic profiling of human decidual immune proteins during Toxoplasma gondii infection.; Authors - Dan Zhang