Anticorps Monoclonal anti-GSTP1

• Phare
• Validé par KD/KO

GSTP1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat et plus (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(8)

CloneNo.

2D4A6

N° de cat : 66715-1-Ig

Synonymes

2D4A6, EC:2.5.1.18, FAEES3, Glutathione S-transferase P, GST3

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Galerie de données de validation

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  WB analysis using 66715-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66715-1-Ig (GSTP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66715-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66715-1-Ig (GSTP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66715-1-Ig

  WB result of GSTP1 antibody (66715-1-Ig; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSTP1 transfected HeLa cells.

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  IP experiment of HeLa using 66715-1-Ig

  IP result of anti-GSTP1 (IP:66715-1-Ig, 4ug; Detection:66715-1-Ig 1:4000) with HeLa cells lysate 1640 ug.

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  IHC staining of human colon cancer using 66715-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66715-1-Ig (GSTP1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 66715-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66715-1-Ig (GSTP1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66715-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66715-1-Ig (GSTP1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 66715-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66715-1-Ig (GSTP1 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 66715-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66715-1-Ig (GSTP1 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 66715-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66715-1-Ig (GSTP1 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 66715-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66715-1-Ig (GSTP1 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 66715-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using GSTP1 antibody (66715-1-Ig, Clone: 2D4A6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HepG2 using 66715-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GSTP1 antibody (66715-1-Ig, Clone: 2D4A6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HuH-7, cellules Caco-2, cellules HCT 116, cellules HEK-293, cellules HeLa, cellules HepG2, cellules Jurkat
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer du col de l'utérus humain, tissu de tumeur ovarienne humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

66715-1-Ig cible GSTP1 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GSTP1 Protéine recombinante Ag8880
• Nom complet: glutathione S-transferase pi 1
• Masse moléculaire calculée: 210 aa, 23 kDa
• Poids moléculaire observé: 23 kDa
• Numéro d’acquisition GenBank: BC010915
• Symbole du gène: GSTP1
• Identification du gène (NCBI): 2950
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Biochem Pharmacol; PMID: 32535103; Authors - Qi Feng ; KD Validated
human	WB	Mol Med Rep; Targeted reversal of multidrug resistance in ovarian cancer cells using exosome‑encapsulated tetramethylpyrazine; Authors - Chenge Zhao
human	IF	Phytomedicine; Natural small-molecules reverse Xeroderma Pigmentosum Complementation Group C (XPC) deficient-mediated drug-resistance in renal cell carcinoma; Authors - Ruihong Chen
mouse	CoIP	Eur J Pharmacol; N6022 attenuates cerebral ischemia/reperfusion injury-induced microglia ferroptosis by promoting Nrf2 nuclear translocation and inhibiting the GSNOR/GSTP1 axis; Authors - Wan-Li Duan
Human		Onco Targets Ther; Mechanism of microRNA-152-3p-Mediated Regulation of Autophagy and Sensitivity in Paclitaxel-Resistant Ovarian Cancer Cells; Authors - Di Wu
human	WB	Bioorg Chem; Sesquiterpene lactones from Elephantopus scaber exhibit cytotoxic effects on glioma cells by targeting GSTP1; Authors - Qiu-Lin Yan

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