Anticorps Polyclonal de lapin anti-GSTT1

• Phare
• Validé par KD/KO

GSTT1 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 15838-1-AP

Synonymes

Glutathione transferase T1 1, GST class theta 1, GSTT1

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Galerie de données de validation

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  WB analysis of human brain using 15838-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15838-1-AP (GSTT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15838-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15838-1-AP (GSTT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 15838-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15838-1-AP (GSTT1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 15838-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15838-1-AP (GSTT1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 15838-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 15838-1-AP (GSTT1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 15838-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15838-1-AP (GSTT1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 15838-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15838-1-AP (GSTT1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 15838-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15838-1-AP (GSTT1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 15838-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15838-1-AP (GSTT1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 15838-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15838-1-AP (GSTT1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 15838-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15838-1-AP (GSTT1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 15838-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15838-1-AP (GSTT1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 15838-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15838-1-AP (GSTT1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 15838-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15838-1-AP (GSTT1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 15838-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15838-1-AP (GSTT1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 15838-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15838-1-AP (GSTT1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 15838-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15838-1-AP (GSTT1 antibody) at dilution of 1:50 (under 10x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, cellules HepG2, tissu cérébral de souris, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IHC: tissu rénal humain, tissu cardiaque humain, tissu cutané humain, tissu pulmonaire humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 4 publications below

Informations sur le produit

15838-1-AP cible GSTT1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GSTT1 Protéine recombinante Ag8588
• Nom complet: glutathione S-transferase theta 1
• Masse moléculaire calculée: 240 aa, 27 kDa
• Poids moléculaire observé: 28 kDa, 30 kDa
• Numéro d’acquisition GenBank: BC007065
• Symbole du gène: GSTT1
• Identification du gène (NCBI): 2952
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GSTT1 belongs to the GST superfamily and Theta family. It is a conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. GSTT1 acts on 1,2-epoxy-3-(4-nitrophenoxy)propane, phenethylisothiocyanate 4-nitrobenzyl chloride and 4-nitrophenethyl bromide. It displays glutathione peroxidase activity with cumene hydroperoxide. GSTM1 and GSTT1 has been suggested as a risk factor in various cancers. GSTT1 is important in the detoxification of unidentified xenobiotics in the large intestine.

Publications

Species	Application	Title
		Cell Syst; Rare Disease Mechanisms Identified by Genealogical Proteomics of Copper Homeostasis Mutant Pedigrees.; Authors - Stephanie A Zlatic
mouse	WB	FASEB J; Gstm1/Gstt1 is essential for reducing cisplatin ototoxicity in CBA/CaJ mice.; Authors - Peipei Li ; KO Validated
hamster	IHC	Toxicol Sci; Determination of Hepatotoxicity and Its Underlying Metabolic Basis of 1,2-dichloropropane in Male Syrian Hamsters and B6C3F1 Mice.; Authors - Min Wei
human	WB	Drug Metab Dispos; Evaluation of hepatic glutathione transferase Mu 1 and Theta 1 activities in humans and mice using genotype information.; Authors - Arakawa Shingo S
human	WB	Curr Issues Mol Biol; COPS5 Conferred the Platinum Resistance in Epithelial Ovarian Cancer; Authors - Ming-Wei Lin
mouse	IHC	Int J Clin Exp Pathol; Different carcinogenic process in cholangiocarcinoma cases epidemically developing among workers of a printing company in Japan.; Authors - Yasunori Sato