Anticorps Polyclonal de lapin anti-GSTZ1

GSTZ1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 14889-1-AP

Synonymes

glutathione transferase zeta 1, GSTZ1, GSTZ1 1, MAAI, MAI, Maleylacetoacetate isomerase

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Galerie de données de validation

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  WB analysis using 14889-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14889-1-AP (GSTZ1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 14889-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14889-1-AP (GSTZ1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 14889-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 14889-1-AP (GSTZ1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 14889-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 14889-1-AP (GSTZ1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IHC: tissu hépatique de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 1 publications below

Informations sur le produit

14889-1-AP cible GSTZ1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GSTZ1 Protéine recombinante Ag6676
• Nom complet: glutathione transferase zeta 1
• Masse moléculaire calculée: 24 kDa
• Poids moléculaire observé: 25-30 kDa
• Numéro d’acquisition GenBank: BC001453
• Symbole du gène: GSTZ1
• Identification du gène (NCBI): 2954
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Glutathione transferase zeta 1(GSTZ1) catalyzes the biotransformation of a range of α-haloacids, including dichloroacetic acid (DCA), and the penultimate step in the tyrosine degradation pathway. GSTZ1 is preferentially expressed in hepatocytes and renal proximal tubule cells where phenylalanine and tyrosine are catabolized(PMID: 16550164). This protein has 3 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	IHC	Front Immunol; Identification and validation of a tyrosine metabolism-related prognostic prediction model and characterization of the tumor microenvironment infiltration in hepatocellular carcinoma; Authors - Yangying Zhou
mouse	WB	J Gerontol A Biol Sci Med Sci; PMID: 24285747; Authors - Rojanathammanee Lalida L
mouse	WB	Age (Dordr); PMID: 25001375; Authors - Lalida Rojanathammanee
rat	WB	BMC Complement Med Ther; Integrated proteomics and metabolomics analysis of lumbar in a rat model of osteoporosis treated with Gushukang capsules; Authors - Ruohui Lin
human	WB	ACS Chem Biol; Chemical Proteomics with Novel Fully Functionalized Fragments and Stringent Target Prioritization Identifies the Glutathione-Dependent Isomerase GSTZ1 as a Lung Cancer Target; Authors - Yi Liao
human	WB	Anal Chem; Spike-In Proteome Enhances Data-Independent Acquisition for Thermal Proteome Profiling; Authors - Qiqi Wang

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