Anticorps Polyclonal de lapin anti-TFIIE Beta

TFIIE Beta Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 11596-1-AP

Synonymes

GTF2E2, TFIIE-beta, TFIIE B, TF2E2

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Galerie de données de validation

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  WB analysis of K-562 using 11596-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11596-1-AP (TFIIE beta antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 11596-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 11596-1-AP (TFIIE beta antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 11596-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11596-1-AP (TFIIE beta antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of A375 using 11596-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A375 cells using TFIIE Beta antibody (11596-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules A375, cellules Jurkat
• Résultats positifs en IF/ICC: cellules A375,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

11596-1-AP cible TFIIE Beta dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TFIIE Beta Protéine recombinante Ag2136
• Nom complet: general transcription factor IIE, polypeptide 2, beta 34kDa
• Masse moléculaire calculée: 291 aa, 33 kDa
• Poids moléculaire observé: 34 kDa
• Numéro d’acquisition GenBank: BC030572
• Symbole du gène: GTF2E2
• Identification du gène (NCBI): 2961
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The general transcription factor IIE (TFIIE) is an essential component of the eukaryotic RNA polymerase II initiation complex, which plays pivotal roles in initiation of protein-coding gene transcription (PMID: 7829083). Transcription initiation factor IIE comprises two subunits, TFIIE-alpha and TFIIE-beta, two chains of which together form a tetramer. TFIIE-beta is encoded by GTF2E2 gene and contains TFIIE beta-DNA binding domain. It mainly functions to recruit TFIIH to the initiation complex and stimulate RNA polymerase II kinase activity (PMID: 10792464).

Publications

Species	Application	Title
mouse	WB	Nucleic Acids Res; Reverse engineering a mouse embryonic stem cell-specific transcriptional network reveals a new modulator of neuronal differentiation.; Authors - De Cegli Rossella R
human	WB	Cancer Gene Ther; GTF2E2 downregulated by miR-340-5p inhibits the malignant progression of glioblastoma; Authors - Xiaolong Qiao

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