Anticorps Polyclonal de lapin anti-TFII I

TFII I Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 10499-1-AP

Synonymes

BAP 135, BAP135, BTK associated protein 135, BTKAP1, DIWS, GTF2I, GTFII I, IB291, SPIN, SRF Phox1 interacting protein, TFII I, WBS, WBSCR6

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Galerie de données de validation

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  WB analysis using 10499-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10499-1-AP (TFII I antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10499-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10499-1-AP (TFII I antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 10499-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10499-1-AP (TFII I antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10499-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10499-1-AP (TFII I antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 10499-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10499-1-AP (TFII I antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary tumor using 10499-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10499-1-AP (TFII I antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 10499-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10499-1-AP (TFII I antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 10499-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10499-1-AP (TFII I antibody at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 10499-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 10499-1-AP (TFII I antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 10499-1-AP

  Immunofluorescent analysis of Hela cells, using GTF2I antibody 10499-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of HeLa using 10499-1-AP

  1X10^6 HeLa cells were stained with 0.2ug TFII I antibody (10499-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules Jurkat
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

10499-1-AP cible TFII I dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TFII I Protéine recombinante Ag0773
• Nom complet: general transcription factor II, i
• Masse moléculaire calculée: 112 kDa
• Poids moléculaire observé: 135 kDa
• Numéro d’acquisition GenBank: BC004472
• Symbole du gène: GTF2I
• Identification du gène (NCBI): 2969
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The GTF2I gene encodes a transcription factor. It negatively regulates agonist-induced calcium entry into cells by interfering with expression of the cation channel TRPC3 at the plasma membrane [PMID: 17023658]. As a transcription factor and/or as a regulator of intracellular calcium levels, GTF2I may play a role in the molecular basis of anxiety[PMID: 22578324]. It interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Required for the formation of functional ARID3A DNA-binding complexes and for activation of immunoglobulin heavy-chain transcription upon B-lymphocyte activation [PMID: 16738337].

Publications

Species	Application	Title
human	WB	Front Cell Dev Biol; Increased Nuclear Transporter Importin 7 Contributes to the Tumor Growth and Correlates With CD8 T Cell Infiltration in Cervical Cancer; Authors - Jing Chen
mouse	WB	Nature; Post-translational control of beige fat biogenesis by PRDM16 stabilization; Authors - Qiang Wang