Anticorps Polyclonal de lapin anti-TFII I

TFII I Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 10499-1-AP

Synonymes

BTKAP1, BTK associated protein 135, Bruton tyrosine kinase-associated protein 135, BAP-135, BAP135

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Galerie de données de validation

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  WB analysis using 10499-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10499-1-AP (TFII I antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10499-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10499-1-AP (TFII I antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10499-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10499-1-AP (TFII I antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 10499-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10499-1-AP (TFII I antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human thyroid cancer using 10499-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10499-1-AP (TFII I antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 10499-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10499-1-AP (TFII I antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 10499-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10499-1-AP (TFII I antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse small intestine using 10499-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 10499-1-AP (TFII I antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 10499-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 10499-1-AP (TFII I antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat small intestine using 10499-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 10499-1-AP (TFII I antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10499-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10499-1-AP (TFII I antibody at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 10499-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 10499-1-AP (TFII I antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 10499-1-AP

  Immunofluorescent analysis of Hela cells, using GTF2I antibody 10499-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules Jurkat
• Résultats positifs en IHC: tissu de cancer de la thyroïde humain, tissu de cancer du côlon humain, tissu de carcinome urothélial humain, tissu d'intestin grêle de rat, tissu d'intestin grêle de souris, tissu rénal de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

10499-1-AP cible TFII I dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TFII I Protéine recombinante Ag0773
• Nom complet: general transcription factor II, i
• Masse moléculaire calculée: 112 kDa
• Poids moléculaire observé: 135 kDa
• Numéro d’acquisition GenBank: BC004472
• Symbole du gène: TFII I
• Identification du gène (NCBI): 2969
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The GTF2I gene encodes a transcription factor. It negatively regulates agonist-induced calcium entry into cells by interfering with expression of the cation channel TRPC3 at the plasma membrane [PMID: 17023658]. As a transcription factor and/or as a regulator of intracellular calcium levels, GTF2I may play a role in the molecular basis of anxiety[PMID: 22578324]. It interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Required for the formation of functional ARID3A DNA-binding complexes and for activation of immunoglobulin heavy-chain transcription upon B-lymphocyte activation [PMID: 16738337].

Publications

Species	Application	Title
human	WB	Front Cell Dev Biol; Increased Nuclear Transporter Importin 7 Contributes to the Tumor Growth and Correlates With CD8 T Cell Infiltration in Cervical Cancer; Authors - Jing Chen
mouse	WB	Nature; Post-translational control of beige fat biogenesis by PRDM16 stabilization; Authors - Qiang Wang
mouse	IF	iScience; Characterizing the differential distribution and targets of Sumo1 and Sumo2 in the mouse brain; Authors - Terry R Suk

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