Anticorps Polyclonal de lapin anti-GUCY1A3

GUCY1A3 Polyclonal Antibody for IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 12605-1-AP

Synonymes

GC SA3, GCS alpha 1, GCS alpha 3, GUC1A3, GUCA3, GUCSA3, GUCY1A1, GUCY1A3

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Galerie de données de validation

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  WB analysis of human brain using 12605-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12605-1-AP (GUCY1A3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 12605-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12605-1-AP (GUCY1A3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 12605-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12605-1-AP (GUCY1A3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 12605-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12605-1-AP (GUCY1A3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human cerebellum using 12605-1-AP

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 12605-1-AP (GUCY1A3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 12605-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12605-1-AP (GUCY1A3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse lung using 12605-1-AP

  IP Result of anti-GUCY1A3 (IP:12605-1-AP, 4ug; Detection:12605-1-AP 1:500) with mouse lung tissue lysate 3600ug.

• 

  IHC staining of human heart using 12605-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12605-1-AP (GUCY1A3 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 12605-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12605-1-AP (GUCY1A3 Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, tissu de cervelet humain, tissu pulmonaire de souris, tissu rénal humain
• Résultats positifs en IP: tissu pulmonaire de souris
• Résultats positifs en IHC: tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

12605-1-AP cible GUCY1A3 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GUCY1A3 Protéine recombinante Ag3293
• Nom complet: guanylate cyclase 1, soluble, alpha 3
• Masse moléculaire calculée: 690 aa, 77 kDa
• Poids moléculaire observé: 77 kDa
• Numéro d’acquisition GenBank: BC028384
• Symbole du gène: GUCY1A3
• Identification du gène (NCBI): 2982
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	IF	Hepatology; Single-cell transcriptomic analysis reveals a hepatic stellate cell-activation roadmap and myofibroblast origin during liver fibrosis.; Authors - Wu Yang
mouse	WB	Nat Commun; Aortic disease in Marfan syndrome is caused by overactivation of sGC-PRKG signaling by NO.; Authors - Andrea de la Fuente-Alonso
mouse	WB	Int J Mol Sci; Hypertensive Effect of Downregulation of the Opioid System in Mouse Model of Different Activity of the Endogenous Opioid System.; Authors - Dominik S Skiba
mouse	WB,IHC	Sci Rep; Hypertension reduces soluble guanylyl cyclase expression in the mouse aorta via the Notch signaling pathway.; Authors - Catarina Rippe
	WB,IF	J Exp Med; Activating NO-sGC crosstalk in the mouse vascular niche promotes vascular integrity and mitigates acute lung injury; Authors - Hao He
mouse	WB	Front Pharmacol; Stimulation of soluble guanylate cyclase by vericiguat reduces skeletal muscle atrophy of mice following chemotherapy; Authors - Bo-Ang Hu