Anticorps Polyclonal de lapin anti-GUCY1B3

• Phare
• Validé par KD/KO

GUCY1B3 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 19011-1-AP

Synonymes

GC S beta 1, GC SB3, GCS beta 1, GCS beta 3, GUC1B3, GUCB3, GUCSB3, GUCY1B1, GUCY1B3

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Galerie de données de validation

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  WB analysis using 19011-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19011-1-AP (GUCY1B3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 19011-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 19011-1-AP (GUCY1B3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 19011-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 19011-1-AP (GUCY1B3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of human placenta using 19011-1-AP

  IP result of anti-GUCY1B3 (IP:19011-1-AP, 4ug; Detection:19011-1-AP 1:500) with human placenta tissue lysate 2600 ug.

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  IHC staining of mouse brain using 19011-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19011-1-AP (GUCY1B3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 19011-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19011-1-AP (GUCY1B3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 19011-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19011-1-AP (GUCY1B3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 19011-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19011-1-AP (GUCY1B3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 19011-1-AP

  Immunofluorescent analysis of Hela cells, using GUCY1B3 antibody 19011-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  IF Staining of MCF-7 using 19011-1-AP

  Immunofluorescent analysis of MCF-7 cells, using GUCY1B3 antibody 19011-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: tissu placentaire humain, tissu pulmonaire de rat, tissu pulmonaire de souris
• Résultats positifs en IP: tissu placentaire humain,
• Résultats positifs en IHC: tissu cérébral de souris, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa, cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 2 publications below

Informations sur le produit

19011-1-AP cible GUCY1B3 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GUCY1B3 Protéine recombinante Ag5196
• Nom complet: guanylate cyclase 1, soluble, beta 3
• Masse moléculaire calculée: 71 kDa
• Poids moléculaire observé: 67-71 kDa
• Numéro d’acquisition GenBank: BC047620
• Symbole du gène: GUCY1B3
• Identification du gène (NCBI): 2983
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Nat Commun; Aortic disease in Marfan syndrome is caused by overactivation of sGC-PRKG signaling by NO.; Authors - Andrea de la Fuente-Alonso
mouse	WB,IHC	Clin Sci (Lond); Empagliflozin prevents cardiomyopathy via sGC-cGMP-PKG pathway in type 2 diabetes mice.; Authors - Mei Xue ; KD Validated
mouse	WB,IHC	Sci Rep; Hypertension reduces soluble guanylyl cyclase expression in the mouse aorta via the Notch signaling pathway.; Authors - Catarina Rippe
mouse	WB	Cell Tissue Res; Cavin-3 (PRKCDBP) deficiency reduces the density of caveolae in smooth muscle.; Authors - Baoyi Zhu
rat	WB	World Neurosurg; Upregulation of Connexin 40 Mediated by Nitric Oxide Attenuates Cerebral Vasospasm After Subarachnoid Hemorrhage via the Nitric Oxide-Cyclic Guanosine Monophosphate-Protein Kinase G Pathway.; Authors - Shi Hai Lan
human	WB	Front Endocrinol (Lausanne); Specific dilation pattern in placental circulation and the NO/sGC role in preeclampsia placental vessels; Authors - Jiaqi Tang