Anticorps Polyclonal de lapin anti-GUF1

• Phare
• Validé par KD/KO

GUF1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

N° de cat : 17489-1-AP

Synonymes

Translation factor GUF1, mitochondrial, Ribosomal back-translocase, GTPase GUF1, Elongation factor 4 homolog, EF-4

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Galerie de données de validation

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  WB analysis of HepG2 using 17489-1-AP

  WB result of GUF1 antibody (17489-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GUF1 transfected HepG2 cells.

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  WB analysis using 17489-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17489-1-AP (GUF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17489-1-AP

  Hela cells were subjected to SDS PAGE followed by western blot with 17489-1-AP (GUF1 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 17489-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17489-1-AP (GUF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17489-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17489-1-AP (GUF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 17489-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 17489-1-AP (GUF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 17489-1-AP

  IP result of anti-GUF1 (IP:17489-1-AP, 3ug; Detection:17489-1-AP 1:500) with HeLa cells lysate 3200ug.

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  IHC staining of human oesophagus using 17489-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human oesophagus using 17489-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung cancer using 17489-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 17489-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung cancer using 17489-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 17489-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 17489-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 17489-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 17489-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 17489-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human oesophagus using 17489-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human oesophagus using 17489-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 17489-1-AP

  Immunofluorescent analysis of HepG2 cells, using GUF1 antibody 17489-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, tissu cérébral de souris, tissu pancréatique de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu œsophagien humain, tissu de cancer du poumon humain, tissu rénal humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

17489-1-AP cible GUF1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GUF1 Protéine recombinante Ag11579
• Nom complet: GUF1 GTPase homolog (S. cerevisiae)
• Masse moléculaire calculée: 669 aa, 74 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC036768
• Symbole du gène: GUF1
• Identification du gène (NCBI): 60558
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GUF1 (GUF1 GTPase), also known as EF-4 (elongation factor 4 homolog), is a 669 amino acid protein belonging to the GTP-binding elongation factor family and the LepA subfamily. Localizing to the mitochondrion inner membrane, GUF1 promotes mitochondrial protein synthesis and binds to mitochondrial ribosomes in a GTP-dependent manner. GUF1 may act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. In western blotting, we got double bands 74 kDa and 70 kDa, due to the N-terminal 1-49 amino acid sequence that could be cut down.

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