Anticorps Polyclonal de lapin anti-Glycophorin A

Glycophorin A Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

N° de cat : 15874-1-AP

Synonymes

CD235a, Glycophorin A, GPA, GPErik, GpMiIII, GPSAT, GYPA, HGpMiIII, HGpMiV, HGpMiX, HGpMiXI, HGpSta(C), MN, MN sialoglycoprotein, MNS, PAS 2, Sialoglycoprotein alpha

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Galerie de données de validation

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  WB analysis of K-562 using 15874-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15874-1-AP (Glycophorin A antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 15874-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15874-1-AP (Glycophorin A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human spleen using 15874-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 15874-1-AP (Glycophorin A antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human spleen using 15874-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 15874-1-AP (Glycophorin A antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon cancer using 15874-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15874-1-AP (Glycophorin A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 15874-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15874-1-AP (Glycophorin A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 15874-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 15874-1-AP (Glycophorin A antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 15874-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 15874-1-AP (Glycophorin A antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human placenta using 15874-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using Glycophorin A antibody (15874-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of human placenta using 15874-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using Glycophorin A antibody (15874-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of K-562 using 15874-1-AP

  1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human Glycophorin A (15874-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules K-562,
• Résultats positifs en IHC: tissu splénique humain, tissu de cancer du côlon humain, tissu pulmonaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu placentaire humain,
• Résultats positifs en cytométrie: cellules K-562,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:300-1:1200
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

15874-1-AP cible Glycophorin A dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Glycophorin A Protéine recombinante Ag8635
• Nom complet: glycophorin A (MNS blood group)
• Masse moléculaire calculée: 150 aa, 16 kDa
• Poids moléculaire observé: 36-38 kDa
• Numéro d’acquisition GenBank: BC005319
• Symbole du gène: GYPA
• Identification du gène (NCBI): 2993
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Glycophorin A (GYPA) is the major transmembrane sialoglycoprotein in erythrocytes. It is a dimeric type I transmembrane protein carrying 15 closely clustered O-linked tetrasaccharides capped with sialic acid/N-acetylneuraminic acid (Neu5Ac). This 36 kDa protein represents the major sialoglycoprotein of the red blood cell membrane displaying about one million copies per cell. (PMID: 9490702)

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C