Anticorps Polyclonal de lapin anti-Granzyme A

Granzyme A Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 11288-1-AP

Synonymes

CTL tryptase, CTLA3, Fragmentin 1, GrA, Granzyme 1, Granzyme A, GZMA, H factor, Hanukkah factor, HF, HFSP

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Galerie de données de validation

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  WB analysis using 11288-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11288-1-AP (Granzyme A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 11288-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11288-1-AP (Granzyme A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 11288-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 11288-1-AP (Granzyme A antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lymphoma using 11288-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 11288-1-AP (Granzyme A antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lymphoma using 11288-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11288-1-AP (Granzyme A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, tissu rénal de rat
• Résultats positifs en IHC: tissu de lymphome humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• FC: See 1 publications below

Informations sur le produit

11288-1-AP cible Granzyme A dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Granzyme A Protéine recombinante Ag1818
• Nom complet: granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3)
• Masse moléculaire calculée: 29 kDa
• Poids moléculaire observé: 30, 60 kDa
• Numéro d’acquisition GenBank: BC015739
• Symbole du gène: GZMA
• Identification du gène (NCBI): 3001
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Granzyme A(GrA, a tryptase) is necessary for target cell lysis in cell-mediated immune responses. It activates mitochondrial outer membrane permeabilization- and caspase-independent cell death pathways by cleaving the mitochondrial complex I component NDUFS3after lys56. Among serine proteases GrA has an unique quaternary structure consisting of a disulphide-linked homodimer of 60kDa linked via Cys93.(PMID: 12819769) This protein has 2 isoforms produced by alternative promoter usage with the MW of 29 and 27 kDa.

Publications

Species	Application	Title
mouse	IHC	Int J Biol Sci; Rapamycin and Low-dose IL-2 Mediate an Immunosuppressive Microenvironment to Inhibit Benign Prostatic Hyperplasia; Authors - Tianyu Cao
human	WB	J Proteomics; Proteomic profiling of human decidual immune proteins during Toxoplasma gondii infection.; Authors - Dan Zhang
human	IHC	Immunopharmacol Immunotoxicol; Identification of discrepancy between CTLA4 expression and CTLA4 activation in gastric cancer.; Authors - Zhen Xiang
chicken	WB	Res Vet Sci; Characterization of cytotoxicity-related gene expression in response to virulent Marek's disease virus infection in the bursa of Fabricius.; Authors - Cui-Ying Chen
chicken	WB,IF,FC	Dev Comp Immunol; Perforin and granzyme A release as novel tool to measure NK cell activation in chickens; Authors - Adil Ijaz