Anticorps Monoclonal anti-Galectin-3

• Phare
• Validé par KD/KO

Galectin-3 Monoclonal Antibody for IF/ICC, IF-P, FC (Intra)

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

IF/ICC, IF-P, FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

Publications(1)

CloneNo.

1C1B2

N° de cat : CL488-60207

Synonymes

Gal 3, CBP35, CBP 35, Carbohydrate-binding protein 35, 35 kDa lectin

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Galerie de données de validation

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  IF Staining of human thyroid cancer using CL488-60207

  Immunofluorescent analysis of (4% PFA) fixed human thyroid cancer tissue using CL488-60207 (Galectin-3 antibody) at dilution of 1:50.

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  IF Staining of human thyroid cancer using CL488-60207

  Immunofluorescent analysis of (4% PFA) fixed human thyroid cancer tissue using CL488-60207 (Galectin-3 antibody) at dilution of 1:50.

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  IF Staining of HeLa using CL488-60207

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 Galectin-3 antibody (CL488-60207, Clone: 1C1B2 ) at dilution of 1:200.

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  FC experiment of HeLa using CL488-60207

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Galectin-3 (CL488-60207, Clone:1C1B2) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF-P: tissu de cancer de la thyroïde humain,
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

CL488-60207 cible Galectin-3 dans les applications de IF/ICC, IF-P, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Galectin-3 Protéine recombinante Ag6753
• Nom complet: lectin, galactoside-binding, soluble, 3
• Masse moléculaire calculée: 26 kDa
• Numéro d’acquisition GenBank: BC001120
• Symbole du gène: Galectin-3
• Identification du gène (NCBI): 3958
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-3, a member of the β-galactoside-binding proteins, contains one carbohydrate recognition domain (CRD) and a proline- and glycine-rich N-terminal domain through which is able to form oligomers (PMID: 14758078). Galectin-3 is widely expressed in many normal tissues and a variety of tumors. It is found intracellularly in nucleus and cytoplasm or secreted outside of cell, being present on the cell surface or in the extracellular space (PMID: 16478649). Galectin-3 is involved in various biological processes including cell growth, adhesion, differentiation, apoptosis, angiogenesis, immune response, neoplastic transformation and metastasis (PMID: 16478649; 14758078).

Publications

Species	Application	Title
mouse	IF	Redox Biol; Myeloid ACE2 protects against septic hypotension and vascular dysfunction through Ang-(1-7)-Mas-mediated macrophage polarization; Authors - Jia-Xin Li

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