Anticorps Polyclonal de lapin anti-Giantin

Giantin Polyclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

6

Avis (1)

N° de cat : 22270-1-AP

Synonymes

Cell and organelle markers, GCP, GCP372, giantin, GOLGB1, Golgi marker, Golgin subfamily B member 1, GOLIM1, Macrogolgin

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Galerie de données de validation

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  WB analysis using 22270-1-AP

  Various lysates were subjected to Tris-Acetate gel system followed by western blot with 22270-1-AP (Giantin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 22270-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Giantin antibody (22270-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 22270-1-AP

  Immunofluorescent analysis of HepG2 cells using 22270-1-AP (Giantin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 22270-1-AP

  Immunofluorescent analysis of HeLa cells using 22270-1-AP (Giantin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa
• Résultats positifs en IF: cellules HepG2, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 4 publications below

Informations sur le produit

22270-1-AP cible Giantin dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, singe
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: golgin B1, golgi integral membrane protein
• Masse moléculaire calculée: 376 kDa
• Poids moléculaire observé: 376 kDa
• Numéro d’acquisition GenBank: NM_004487
• Symbole du gène: GOLGB1
• Identification du gène (NCBI): 2804
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Giantin is a conserved Golgi membrane protein containing a large cytoplasmic domain. It may function to form the intercisternal cross-bridges of the Golgi complex. Anti-Giantin has been widely used to label the Golgi apparatus.

Publications

Species	Application	Title
human	IF	iScience; Cytosolic Ca2+ Modulates Golgi Structure Through PKCα-Mediated GRASP55 Phosphorylation.; Authors - Stephen Ireland
human		Int J Nanomedicine; PMID: 28769563; Authors - Huiying Xue
human	IF	J Alzheimers Dis; High Fat Diet Mediates Amyloid-β Cleaving Enzyme 1 Phosphorylation and SUMOylation, Enhancing Cognitive Impairment in APP/PS1 Mice; Authors - Jian Bao
human	WB	Methods Mol Biol; Immunofluorescence Microscopy of the Mammalian Golgi Apparatus; Authors - Maryam Arab
monkey	IF	Seizure; Heterozygous RELN missense variants associated with genetic generalized epilepsy; Authors - Xiaoling Wu
human	IF	Nat Methods; StayGold variants for molecular fusion and membrane-targeting applications; Authors - Ryoko Ando

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Boyan (Verified Customer) (04-28-2023)

Detected a band around the expected size, however the signal was really weak.

• Applications: Western Blot