Anticorps Monoclonal anti-Glucagon

Glucagon Monoclonal Antibody for IF, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

CloneNo.

2A8G1

N° de cat : 67286-1-Ig

Synonymes

GCG, GLP 2, GLP1, GLP2, glucagon, GRPP, GRPP Oxyntomodulin, OXM

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Galerie de données de validation

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  IHC staining of human pancreas using 67286-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 67286-1-Ig (Glucagon antibody) at dilution of 1:10000(under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas using 67286-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 67286-1-Ig (Glucagon antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse pancreas using 67286-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67286-1-Ig (Glucagon antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 67286-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67286-1-Ig (Glucagon antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 67286-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67286-1-Ig (Glucagon antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human pancreas using 67286-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human pancreas tissue using 67286-1-Ig (Glucagon antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). The B cells of the islets of Langerhans was labelled in red with 15848-1-AP (Insulin antibody).

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  IF Staining of mouse pancreas using 67286-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using Glucagon antibody (67286-1-Ig, Clone: 2A8G1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), INS antibody (15848-1-AP, red).

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Applications testées

• Résultats positifs en IHC: tissu pancréatique humain, tissu pancréatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu pancréatique humain, tissu pancréatique de souris

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:2000-1:15000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 3 publications below

Informations sur le produit

67286-1-Ig cible Glucagon dans les applications de IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Glucagon Protéine recombinante Ag10629
• Nom complet: glucagon
• Masse moléculaire calculée: 180 aa, 21 kDa
• Numéro d’acquisition GenBank: BC005278
• Symbole du gène: GCG
• Identification du gène (NCBI): 2641
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Glucagon is a 29-amino acid peptide hormone secreted from the pancreatic alpha cells with a powerful stimulatory effect on hepatic glucose production acting to increase plasma glucose levels. Glucagon is best known as the counter-regulatory hormone to INS, and normal glucose homeostasis depends largely on the balanced secretion of INS and glucagon from the pancreatic beta and alpha cells, respectively.The regulation of glucose metabolism by glucagon is mediated by its direct action on the peripheral tissues such as the liver and also by the brain. Glucagon is also released postprandially in a transient manner, which was shown to be involved in inhibition of food intake via reduction of meal size.

Publications

Species	Application	Title
mouse	IF	Food Chem X; Dendrobium officinale leaf polysaccharides ameliorated hyperglycemia and promoted gut bacterial associated SCFAs to alleviate type 2 diabetes in adult mice.; Authors - Jingyu Fang
mouse	IF	Mol Cell Endocrinol; Mof acetyltransferase inhibition ameliorates glucose intolerance and islet dysfunction of type 2 diabetes via targeting pancreatic α-cells.; Authors - Xinghong Guo
mouse	IF	NPJ Biofilms Microbiomes; Bacteroides ovatus accelerates metformin-induced vitamin B12 deficiency in type 2 diabetes patients by accumulating cobalamin; Authors - Manyun Chen
human		Mol Metab; Long non-coding RNA LncCplx2 regulates glucose homeostasis and pancreatic β cell function; Authors - Linlin Wang