Anticorps Monoclonal anti-Glucagon

Glucagon Monoclonal Antibody for IF-P

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

IF-P

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

Publications(1)

CloneNo.

2A8G1

N° de cat : CL488-67286

Synonymes

GCG, GLP 2, GLP1, GLP2, glucagon, GRPP, GRPP Oxyntomodulin, OXM

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Galerie de données de validation

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  IF Staining of mouse pancreas using CL488-67286

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using the CoraLite® Plus 488-conjugated version of this antibody, CL488-67286 (Glucagon antibody) and CL594-66198 (INS antibody), at dilution of 1:400. The B cells of the islets of Langerhans was labelled in red with CL594-66198(INS antibody).

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Applications testées

• Résultats positifs en IF-P: tissu pancréatique de souris,

Dilution recommandée

• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

CL488-67286 cible Glucagon dans les applications de IF-P et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Glucagon Protéine recombinante Ag10629
• Nom complet: glucagon
• Masse moléculaire calculée: 180 aa, 21 kDa
• Numéro d’acquisition GenBank: BC005278
• Symbole du gène: Glucagon
• Identification du gène (NCBI): 2641
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Glucagon is a 29-amino acid peptide hormone secreted from the pancreatic alpha cells with a powerful stimulatory effect on hepatic glucose production acting to increase plasma glucose levels. Glucagon is best known as the counter-regulatory hormone to INS, and normal glucose homeostasis depends largely on the balanced secretion of INS and glucagon fromthe pancreatic beta and alpha cells, respectively.The regulation of glucose metabolism by glucagon is mediated by its direct action on the peripheral tissues such as the liver and also by the brain. Glucagon is also released postprandially in a transient manner, which was shown to be involved in inhibition of food intake via reduction of meal size.

Publications

Species	Application	Title
mouse	IF	iScience; Optimal HSF1 activation in response to acute cold stress in BAT requires nuclear TXNIP; Authors - Althea N Waldhart

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