Anticorps Monoclonal anti-Glutamine Synthetase

• Phare
• Validé par KD/KO

Glutamine Synthetase Monoclonal Antibody for IF-P, FC (Intra)

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris et plus (1)

Applications

IF-P, FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

Publications(2)

CloneNo.

1B6G4

N° de cat : CL488-66323

Synonymes

GLUL, 1B6G4, EC:2.3.1.225, EC:6.3.1.2, GLNS

---

Galerie de données de validation

• 

  IF Staining of mouse brain using CL488-66323

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL488-66323 (Glutamine synthetase antibody) at dilution of 1:50.

• 

  IF Staining of mouse brain using CL488-66323

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 Glutamine Synthetase antibody (CL488-66323-2, Clone: 1B6G4 ) at dilution of 1:200.

• 

  FC experiment of HepG2 using CL488-66323

  1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human Glutamine Synthetase (CL488-66323, Clone:1B6G4) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en IF-P: tissu cérébral de souris,
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

CL488-66323 cible Glutamine Synthetase dans les applications de IF-P, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: poisson-zèbre
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Glutamine Synthetase Protéine recombinante Ag6309
• Nom complet: glutamate-ammonia ligase (glutamine synthetase)
• Masse moléculaire calculée: 374 aa, 42 kDa
• Numéro d’acquisition GenBank: BC011700
• Symbole du gène: Glutamine Synthetase
• Identification du gène (NCBI): 2752
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GLUL(Glutamine synthetase) is also named as GS,GLNS and belongs to the glutamine synthetase family.This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner By similarity. Essential for proliferation of fetal skin fibroblasts(PMID:18662667).Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD).Organismal glutamine production is augmented secondary to an increase in the activity of glutamine synthetase in the lung and skeletal muscle(PMID:7630137). The antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

Publications

Species	Application	Title
zebrafish	IF	J Cell Sci; Iterative Bleaching Extends Multiplexity (IBEX) facilitates simultaneous identification of all major retinal cell types; Authors - Aanandita A Kothurkar
		Methods Mol Biol; Imaging Cell Signaling in Tissues Using the IBEX Method; Authors - Yuxuan Meng

{{ptg:RelatedPrimaryAntibodies}}