Anticorps Recombinant de lapin anti-Glypican 2

Glypican 2 Recombinant Antibody for ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

ELISA

Conjugaison

Non conjugué

CloneNo.

230518A4

N° de cat : 83241-1-RR

Synonymes

GPC2, Secreted glypican-2, FLJ38962, 230518A4

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Galerie de données de validation

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  FC experiment of HeLa using 83241-1-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Gpc2 Recombinant Antibody (83241-1-RR, Clone:230518A4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of SH-SY5Y using 83241-1-RR

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug Gpc2 Recombinant Antibody (83241-1-RR, Clone:230518A4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83241-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83241-1-RR against Human Glypican 2 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en FC (Intra): cellules SH-SY5Y, cellules HeLa

Dilution recommandée

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83241-1-RR cible Glypican 2 dans les applications de ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Glypican 2 Protéine recombinante Ag4041
• Nom complet: glypican 2
• Masse moléculaire calculée: 579 aa, 63 kDa
• Poids moléculaire observé: 62，70-100 kDa
• Numéro d’acquisition GenBank: BC027972
• Symbole du gène: GPC2
• Identification du gène (NCBI): 221914
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GPC2 (glypican 2), which is expected to be located in cell membrane and extracellular space. It is expressed in lymphoid tissue, skin and testis. The calculated molecular weight of the protein is 62 kDa. GPC2 is mainly active in growing nervous tissues and thyroid cancer tissues (PMID: 28616017). It participates in the growth and differentiation of neuronal axons. Increasing evidence has demonstrated the overexpression of GPC2 in neuroblastoma, a kind of childhood cancer. There is a discovery that GPC2 can be employed as a diagnostic, prognostic, and immunological predictor of generalized cancers. The study may broaden the train of thought toward application of GPC2 in immunotherapy (PMID: 35345673).

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