Anticorps Recombinant de lapin anti-Granzyme B

Granzyme B Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC (Intra)

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

240761G4

N° de cat : CL647-98070

Synonymes

GZMB, 240761G4, Cathepsin G-like 1, CCPI, CGL 1

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Galerie de données de validation

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  FC experiment of human PBMCs using CL647-98070

  1x10^6 human PBMCs were intracellularly stained with FITC Plus Anti-Human CD8a, and 5 ul CoraLite® Plus 647 Anti-Human Granzyme B Rabbit RecAb (CL647-98070, Clone: 240761G4) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of human PBMCs using CL647-98070

  1x10^6 human PBMCs were intracellularly stained with 5 ul CoraLite® Plus 647 Anti-Human Granzyme B Rabbit RecAb (CL647-98070, Clone: 240761G4) (red), or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en FC (Intra): CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-98070 cible Granzyme B dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Granzyme B Protéine recombinante Eg0592
• Nom complet: granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
• Masse moléculaire calculée: 247 aa, 28 kDa
• Numéro d’acquisition GenBank: BC030195
• Symbole du gène: Granzyme B
• Identification du gène (NCBI): 3002
• Conjugaison: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

GZMB (Granzyme B) is also named as CGL1, CSPB, CTLA1, GRB and belongs to the Granzyme subfamily. This enzyme is necessary for target cell lysis in cell-mediated immune responses. The cytotoxic lymphocyte protease granzyme B can promote apoptosis through direct processing and activation of members of the caspase family. Granzyme B can also cleave the BH3-only protein, BID, to promote caspase-independent mitochondrial permeabilization (PMID:17283187). Granzyme B induces lamin B degradation in isolated nuclei less efficiently than Granzyme A (PMID:11331782).

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