Anticorps Polyclonal de lapin anti-H2AFY

H2AFY Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 26875-1-AP

Synonymes

H2AF12M, H2A/y, H2A.y, H2A histone family, member Y, Core histone macro-H2A.1

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Galerie de données de validation

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  WB analysis using 26875-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26875-1-AP (H2AFY antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 26875-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 26875-1-AP (H2AFY Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 26875-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 26875-1-AP (H2AFY Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Caco-2 using 26875-1-AP

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 26875-1-AP (H2AFY Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 26875-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 26875-1-AP (H2AFY Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 26875-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 26875-1-AP (H2AFY antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 26875-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using H2AFY antibody (26875-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules Caco-2, cellules HeLa, cerveau de rat, tissu cérébral de souris
• Résultats positifs en IHC: human intrahepatic cholangiocarcinoma tissue, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

26875-1-AP cible H2AFY dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: H2AFY Protéine recombinante Ag25445
• Nom complet: H2A histone family, member Y
• Masse moléculaire calculée: 372 aa, 40 kDa
• Poids moléculaire observé: 40 kDa
• Numéro d’acquisition GenBank: BC013331
• Symbole du gène: H2AFY
• Identification du gène (NCBI): 9555
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human,mouse	WB,IF,IP	Cell Death Discov; CYB561 promotes HER2+ breast cancer proliferation by inhibiting H2AFY degradation; Authors - Ting Zhao

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