Anticorps Polyclonal de lapin anti-H2AFY2

H2AFY2 Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 17030-1-AP

Synonymes

Core histone macro H2A.2, H2A histone family, member Y2, H2AFY2, histone, Histone macroH2A2, macroH2A2, mH2A2

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Galerie de données de validation

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  WB analysis of HepG2 using 17030-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17030-1-AP (H2AFY2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 17030-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17030-1-AP (H2AFY2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 17030-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 17030-1-AP (H2AFY2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 17030-1-AP

  IP Result of anti-H2AFY2 (IP:17030-1-AP, 4ug; Detection:17030-1-AP 1:500) with HepG2 cells lysate 1000ug.

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Applications testées

• Résultats positifs en WB: cellules HepG2, tissu hépatique humain, tissu rénal de souris
• Résultats positifs en IP: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below

Informations sur le produit

17030-1-AP cible H2AFY2 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: H2AFY2 Protéine recombinante Ag10487
• Nom complet: H2A histone family, member Y2
• Masse moléculaire calculée: 372 aa, 40 kDa
• Poids moléculaire observé: 40 kDa
• Numéro d’acquisition GenBank: BC016172
• Symbole du gène: H2AFY2
• Identification du gène (NCBI): 55506
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	J Cell Physiol; MCPIP1 regulates RORα expression to protect against liver injury induced by lipopolysaccharide via modulation of miR-155.; Authors - Zhenzhen Li
mouse	WB	Cell Physiol Biochem; Klf4 Alleviates Lipopolysaccharide-Induced Inflammation by Inducing Expression of MCP-1 Induced Protein 1 to Deubiquitinate TRAF6.; Authors - Zhenzhen Li
mouse	WB	Immunol Cell Biol; ING4 alleviated lipopolysaccharide-induced inflammation by regulating the NF-κB pathway via a direct interaction with SIRT1.; Authors - Yunshu Yang
mouse	WB	Arch Biochem Biophys; ROR alpha protects against LPS-induced inflammation by down-regulating SIRT1/NF-kappa B pathway.; Authors - Shichao Han
mouse	WB	J Cell Physiol; MCPIP1 alleviated lipopolysaccharide-induced liver injury by regulating SIRT1 via modulation of microRNA-9.; Authors - Shichao Han