Anticorps Monoclonal anti-HA Tag

HA Tag Monoclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Protéine recombinante et plus (2)

Applications

WB, IF/ICC, IP, CoIP, ChIP, RIP, ELISA, PLA

Conjugaison

Non conjugué

Publications(315)

CloneNo.

1F5C6

Avis (1)

N° de cat : 66006-2-Ig

Synonymes

YPYDVPDYA

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• Galerie de données de validation
• Proteintech vs. Other Vendors

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  WB analysis of HEK-293 using 66006-2-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66006-2-Ig (HA Tag antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Ag19172 using 66006-2-Ig

  HA tagged fusion protein was subjected to SDS-PAGE followed by western blot of HA-tag monoclonal antibody 66006-2-Ig at 0.1μg/mL.

• 

  WB analysis of Ag19172 using 66006-2-Ig

  HA tagged fusion protein was subjected to SDS-PAGE followed by western blot of HA-tag monoclonal antibody 66006-2-Ig at 0.05μg/mL.

• 

  WB analysis of Ag19172 using 66006-2-Ig

  HA tagged fusion protein was subjected to SDS-PAGE followed by western blot of HA-tag monoclonal antibody 66006-2-Ig at 0.025μg/mL.

• 

  WB analysis of Ag19172 using 66006-2-Ig

  HA tagged fusion protein was subjected to SDS-PAGE followed by western blot of HA-tag monoclonal antibody 66006-2-Ig at 0.0125μg/mL.

• 

  IP experiment of Transfected HEK-293 using 66006-2-Ig

  IP result of anti-HA Tag (IP:66006-2-Ig, 5ug; Detection:66006-2-Ig 1:2000) with Transfected HEK-293 cells lysate 1600 ug.

• 

  IF Staining of Transfected HEK-293 using 66006-2-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using HA Tag antibody (66006-2-Ig, Clone: 1F5C6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293 transfectées, cellules HEK-293
• Résultats positifs en IP: cellules HEK-293 transfectées,
• Résultats positifs en IF/ICC: cellules HEK-293 transfectées,

Dilution recommandée

• Western Blot (WB): WB : 1:10000-1:100000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 227 publications below
• IF: See 28 publications below
• IP: See 69 publications below
• ELISA: See 1 publications below
• CoIP: See 39 publications below
• ChIP: See 3 publications below
• RIP: See 1 publications below

Informations sur le produit

66006-2-Ig cible HA Tag dans les applications de WB, IF/ICC, IP, CoIP, ChIP, RIP, ELISA, PLA et montre une réactivité avec des échantillons Protéine recombinante

• Réactivité: Protéine recombinante
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: HA Tag
• Symbole du gène: HA Tag
• Identification du gène (NCBI): 6
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Protein tags are a protein or peptide sequences located either on the C- or N- terminal of the target protein, which can facilitate solubility, detection, purification, localization, and expression of the target protein. The HA tag corresponds to amino acid residues YPYDVPDYA of a surface glycoprotein -human influenza virus hemagglutinin (HA). The HA tag is commonly used for a variety of research applications including chromatin immunoprecipitation, ELISA, flow cytometry, western blotting, immunocytochemistry/immunofluorescence among of others.

Publications

Species	Application	Title
	WB	Cell; SND1 binds SARS-CoV-2 negative-sense RNA and promotes viral RNA synthesis through NSP9; Authors - Nora Schmidt
	WB,IP	Cell Host Microbe; Microbiota-derived urocanic acid triggered bytyrosine kinase inhibitors potentiates cancer immunotherapy efficacy; Authors - Mengying Zhang
		Cell Res; Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells.; Authors - Fei Peng
	IP	Nat Commun; Targeting the transcription factor HES1 by L-menthol restores protein phosphatase 6 in keratinocytes in models of psoriasis; Authors - Zhikai Wang
	IP	J Clin Invest; FAM117B promotes gastric cancer growth and drug resistance by targeting the KEAP1/NRF2 signaling pathway; Authors - Yunjiang Zhou
	WB	Nat Microbiol; IFI16 directly senses viral RNA and enhances RIG-I transcription and activation to restrict influenza virus infection.; Authors - Zhimin Jiang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Parijat (Verified Customer) (03-06-2024)

Good antibody for immunofluorescence. Low background.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: 293T Cells

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