Anticorps Polyclonal de lapin anti-HADHA

• Phare
• Validé par KD/KO

HADHA Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

23

N° de cat : 10758-1-AP

Synonymes

78 kDa gastrin binding protein, ECHA, HADH, HADHA, LCHAD, MTPA, TP ALPHA

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Galerie de données de validation

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  WB analysis of HeLa cells using 10758-1-AP

  WB result of HADHA antibody (10758-1-AP, 1:4000) with si-control and si-HADHA transfected HeLa cells.

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  WB analysis using 10758-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10758-1-AP (HADHA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10758-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10758-1-AP (HADHA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10758-1-AP

  IP result of anti-HADHA(IP:10758-1-AP, 4ug; Detection:10758-1-AP 1:10000) with HeLa cells lysate 1080 ug.

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  IHC staining of human heart using 10758-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10758-1-AP (HADHA antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 10758-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10758-1-AP (HADHA antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary using 10758-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 10758-1-AP (HADHA antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 10758-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10758-1-AP (HADHA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10758-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10758-1-AP (HADHA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 10758-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10758-1-AP (HADHA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 10758-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10758-1-AP (HADHA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa cells using 10758-1-AP

  IF result of HADHA antibody (10758-1-AP, 1:50) with si-control and si-HADHA transfected HeLa cells.

• 

  IF Staining of HeLa using 10758-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 10758-1-AP (HADHA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 10758-1-AP

  Immunofluorescent analysis of HepG2 cells, using HADHA antibody 10758-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of HepG2 using 10758-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug HADHA antibody (10758-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HEK-293, cellules HeLa, cellules NIH/3T3, tissu hépatique de rat, tissu hépatique de souris, tissu rénal de rat, tissu rénal de souris
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu cardiaque humain, tissu cardiaque de souris, tissu de cancer du côlon humain, tissu ovarien humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa, cellules HepG2
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 21 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

10758-1-AP cible HADHA dans les applications de WB, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HADHA Protéine recombinante Ag1211
• Nom complet: hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit
• Masse moléculaire calculée: 83 kDa
• Poids moléculaire observé: 70-79 kDa
• Numéro d’acquisition GenBank: BC009235
• Symbole du gène: HADHA
• Identification du gène (NCBI): 3030
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HADHA(Trifunctional enzyme subunit alpha, mitochondrial) is also named as HADH,78 kDa gastrin-binding protein.It belongs to the enoyl-CoA hydratase/isomerase family in the N-terminal section and the 3-hydroxyacyl-CoA dehydrogenase family in the central section.It harbors the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities.Defects in HADHA are a cause of trifunctional protein deficiency (TFP deficiency) and long-chain 3-hydroxyl-CoA dehydrogenase deficiency (LCHAD deficiency) and maternal acute fatty liver of pregnancy (AFLP).

Publications

Species	Application	Title
human	WB	Cell; Mitochondrial Sirtuin Network Reveals Dynamic SIRT3-Dependent Deacetylation in Response to Membrane Depolarization.; Authors - Wen Yang
human	WB	Cell Metab; DNAJC19, a mitochondrial cochaperone associated with cardiomyopathy, forms a complex with prohibitins to regulate cardiolipin remodeling.; Authors - Ricarda Richter-Dennerlein
pig	WB	Sci Total Environ; Protein lysine acetylation played an important role in NH3-induced AEC2 damage and pulmonary fibrosis in piglets; Authors - Xiaotong Wang
mouse	WB	Cell Rep; Feedback repression of PPARα signaling by Let-7 microRNA.; Authors - Tomoki Yagai
mouse	WB	Oxid Med Cell Longev; Mild Hypothermia Attenuates Hepatic Ischemia-Reperfusion Injury through Regulating the JAK2/STAT3-CPT1a-Dependent Fatty Acid β-Oxidation.; Authors - Wei Wang
rat	WB	Biochim Biophys Acta; Succinate accumulation impairs cardiac pyruvate dehydrogenase activity through GRP91-dependent and independent signaling pathways: therapeutic effects of ginsenoside Rb1.; Authors - Jia Li