Anticorps Monoclonal anti-HADHA

• Phare
• Validé par KD/KO

HADHA Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(9)

CloneNo.

3E9B1

N° de cat : 60250-1-Ig

Synonymes

3E9B1, 78 kDa gastrin binding protein, 78 kDa gastrin-binding protein, EC:2.3.1.-, ECHA

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Galerie de données de validation

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  WB analysis of HEK293 cells using 60250-1-Ig

  WB result of HADHA specific antibody (60250-1-Ig, 1:4000) with si-control and si-HADHA transfected HEK293 cells.

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  WB analysis using 60250-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60250-1-Ig (HADHA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 60250-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60250-1-Ig (HADHA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 60250-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60250-1-Ig (HADHA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 60250-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 60250-1-Ig (HADHA antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 60250-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 60250-1-Ig (HADHA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 60250-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 60250-1-Ig (HADHA antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HepG2 using 60250-1-Ig

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 60250-1-Ig(HADHA antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  Affinity and Kinetic Characterization of 60250-1-Ig

  Biolayer interferometry (BLl) kinetic assay of 60250-1-Ig against Human HADHA was performed. The affinity constant is 17.3 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK293, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules NIH/3T3, cellules RAW 264.7
• Résultats positifs en IHC: tissu cardiaque de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 9 publications below

Informations sur le produit

60250-1-Ig cible HADHA dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HADHA Protéine recombinante Ag1211
• Nom complet: hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit
• Masse moléculaire calculée: 83 kDa
• Poids moléculaire observé: 79 kDa
• Numéro d’acquisition GenBank: BC009235
• Symbole du gène: HADHA
• Identification du gène (NCBI): 3030
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HADHA(Trifunctional enzyme subunit alpha, mitochondrial) is also named as HADH,78 kDa gastrin-binding protein.It belongs to the enoyl-CoA hydratase/isomerase family in the N-terminal section and the 3-hydroxyacyl-CoA dehydrogenase family in the central section.It harbors the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities.Defects in HADHA are a cause of trifunctional protein deficiency (TFP deficiency) and long-chain 3-hydroxyl-CoA dehydrogenase deficiency (LCHAD deficiency) and maternal acute fatty liver of pregnancy (AFLP).

Publications

Species	Application	Title
mouse	WB	Commun Biol; A G1528C Hadha knock-in mouse model recapitulates aspects of human clinical phenotypes for long-chain 3-hydroxyacyl-CoA dehydrogenase deficiency; Authors - Garen Gaston
human	WB	Front Oncol; Multi-Omics Analysis of Fatty Acid Metabolism in Thyroid Carcinoma.; Authors - Jinghui Lu
mouse	WB	Proteomics; Quantitative Proteomic Study of Myocardial Mitochondria in Urea Transporter B Knockout Mice.; Authors - Yanwei Du ; KO Validated
human	WB	Cell Biosci; Exosomes from hyperglycemia-stimulated vascular endothelial cells contain versican that regulate calcification/senescence in vascular smooth muscle cells.; Authors - Shuang Li
human	WB	JCI Insight; Mitochondrial bioenergetics and cardiolipin remodeling abnormalities in mitochondrial trifunctional protein deficiency; Authors - Eduardo Vieira Neto
human	WB	J Cancer; Knockdown of HOXD13 in Oral Squamous Cell Carcinoma Inhibited its Proliferation, Migration, and Influenced Fatty Acid Metabolism; Authors - Xingyue Ma

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