Anticorps Polyclonal de lapin anti-HAT1

• Phare
• Validé par KD/KO

HAT1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Conjugaison

Non conjugué

Publications(13)

Avis (2)

N° de cat : 11432-1-AP

Synonymes

EC:2.3.1.48, KAT1

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Galerie de données de validation

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  WB analysis using 11432-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11432-1-AP (HAT1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11432-1-AP

  WB result of HAT1 antibody (11432-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HAT1 transfected HEK-293 cells.

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  IP experiment of HepG2 using 11432-1-AP

  IP result of anti-HAT1 (IP:11432-1-AP, 4ug; Detection:11432-1-AP 1:500) with HepG2 cells lysate 3200ug.

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  IHC staining of human lymphoma using 11432-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 11432-1-AP (HAT1 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of mouse ovary using 11432-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse ovary tissue using HAT1 antibody (11432-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 11432-1-AP

  Immunofluorescent analysis of HepG2 cells, using HAT1 antibody 11432-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules MCF-7, cellules MDA-MB-231, cellules NIH/3T3
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu de lymphome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu ovarien de souris,
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IHC: See 2 publications below
• IF: See 4 publications below

Informations sur le produit

11432-1-AP cible HAT1 dans les applications de WB, IHC, IF/ICC, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HAT1 Protéine recombinante Ag1952
• Nom complet: histone acetyltransferase 1
• Masse moléculaire calculée: 419 aa, 50 kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: BC018682
• Symbole du gène: HAT1
• Identification du gène (NCBI): 8520
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Nature; A druggable copper-signalling pathway that drives inflammation; Authors - Stéphanie Solier
human	WB,IF	Cell Death Dis; HAT1/HDAC2 mediated ACSL4 acetylation confers radiosensitivity by inducing ferroptosis in nasopharyngeal carcinoma; Authors - Peijun Zhou
		Elife; Human DUX4 and mouse Dux interact with STAT1 and broadly inhibit interferon-stimulated gene induction; Authors - Amy E Spens
human	WB	EMBO Rep; Histone acetyltransferase 1 is a succinyltransferase for histones and non-histones and promotes tumorigenesis.; Authors - Guang Yang ; KD Validated
human	WB	Mol Cell Proteomics; Acetylproteomic analysis reveals functional implications of lysine acetylation in human spermatozoa (sperm).; Authors - Heguo Yu
rat	IHC	Cereb Cortex; New Cortical Neurodegenerative Pathways in the Hypertensive Rat Brain.; Authors - Ben Shabat Moti

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kathryn (Verified Customer) (04-22-2022)

This antibody works very well to stain nuclei of developed oocytes in the mouse ovary.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Mouse ovary sections

HAT1 Antibody Immunofluorescence validation (1:100 dilution) in Mouse ovary sections (Cat no:11432-1-AP)

FH

Leonardo (Verified Customer) (05-29-2019)

The antibody works well in a good range of sample (5-40 ug) in both cell lines I tested (MDA-MB-231 and HCC1806). I definitely recommend.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: breast cancer cell lines

HAT1 Antibody Western Blot, validation (1:1000 dilution) in breast cancer cell lines (Cat no:11432-1-AP)

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