Anticorps Polyclonal de lapin anti-HAT1

• Phare
• Validé par KD/KO

HAT1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

9

Avis (2)

N° de cat : 11432-1-AP

Synonymes

HAT1, histone acetyltransferase 1, KAT1

---

Galerie de données de validation

• 

  WB analysis of HEK-293 using 11432-1-AP

  WB result of HAT1 antibody (11432-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HAT1 transfected HEK-293 cells.

• 

  WB analysis using 11432-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11432-1-AP (HAT1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 11432-1-AP

  IP Result of anti-HAT1 (IP:11432-1-AP, 4ug; Detection:11432-1-AP 1:500) with HepG2 cells lysate 3200ug.

• 

  IHC staining of human lymphoma using 11432-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 11432-1-AP (HAT1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HepG2 using 11432-1-AP

  Immunofluorescent analysis of HepG2 cells, using HAT1 antibody 11432-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of mouse ovary using 11432-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse ovary tissue using HAT1 antibody (11432-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules C6, cellules HEK-293, cellules MDA-MB-231, cellules NIH/3T3
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu de lymphome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu ovarien de souris, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Informations sur le produit

11432-1-AP cible HAT1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HAT1 Protéine recombinante Ag1952
• Nom complet: histone acetyltransferase 1
• Masse moléculaire calculée: 419 aa, 50 kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: BC018682
• Symbole du gène: HAT1
• Identification du gène (NCBI): 8520
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Nature; A druggable copper-signalling pathway that drives inflammation; Authors - Stéphanie Solier
human	WB	EMBO Rep; Histone acetyltransferase 1 is a succinyltransferase for histones and non-histones and promotes tumorigenesis.; Authors - Guang Yang ; KD Validated
human	WB	Mol Cell Proteomics; Acetylproteomic analysis reveals functional implications of lysine acetylation in human spermatozoa (sperm).; Authors - Heguo Yu
rat	IHC	Cereb Cortex; New Cortical Neurodegenerative Pathways in the Hypertensive Rat Brain.; Authors - Ben Shabat Moti
mouse	WB	Alcohol; Hepatic COX-2 expression protects mice from an alcohol-high fat diet-induced metabolic disorder by involving protein acetylation related energy metabolism.; Authors - Minjie Chen
human	IF	Biochem Biophys Res Commun; Identification and characterization of an endoplasmic reticulum localization motif.; Authors - Yong-Wei Nie

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Kathryn (Verified Customer) (04-22-2022)

This antibody works very well to stain nuclei of developed oocytes in the mouse ovary.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Mouse ovary sections

HAT1 Antibody Immunofluorescence validation (1:100 dilution) in Mouse ovary sections (Cat no:11432-1-AP)

FH

Leonardo (Verified Customer) (05-29-2019)

The antibody works well in a good range of sample (5-40 ug) in both cell lines I tested (MDA-MB-231 and HCC1806). I definitely recommend.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: breast cancer cell lines

HAT1 Antibody Western Blot, validation (1:1000 dilution) in breast cancer cell lines (Cat no:11432-1-AP)