Anticorps Polyclonal de lapin anti-HAUS4

HAUS4 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 20104-1-AP

Synonymes

C14orf94, HAUS augmin-like complex subunit 4

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Galerie de données de validation

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  WB analysis of human brain using 20104-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20104-1-AP (HAUS4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 20104-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 20104-1-AP (HAUS4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skin using 20104-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 20104-1-AP (HAUS4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 20104-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 20104-1-AP (HAUS4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 20104-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 20104-1-AP (HAUS4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 20104-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 20104-1-AP (HAUS4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 20104-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 20104-1-AP (HAUS4 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human colon cancer using 20104-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20104-1-AP (HAUS4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 20104-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20104-1-AP (HAUS4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 20104-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20104-1-AP (HAUS4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 20104-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20104-1-AP (HAUS4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, tissu cutané de souris, tissu placentaire humain, tissu pulmonaire de souris
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu de cancer du côlon humain, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

20104-1-AP cible HAUS4 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HAUS4 Protéine recombinante Ag13717
• Nom complet: chromosome 14 open reading frame 94
• Masse moléculaire calculée: 363 aa, 42 kDa
• Poids moléculaire observé: 28 kDa, 37 kDa, 42 kDa
• Numéro d’acquisition GenBank: BC002554
• Symbole du gène: C14orf94
• Identification du gène (NCBI): 54930
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HAUS4, also named as C14orf94, has four isoforms with MW 27-29 kDa, 37 kDa and 42 kDa.

Publications

Species	Application	Title
human	WB	Nat Commun; Structural mechanisms for centrosomal recruitment and organization of the microtubule nucleator γ-TuRC; Authors - Qi Gao
human	WB	J Cell Biol; Reconstitution and mechanistic dissection of the human microtubule branching machinery.; Authors - Yaqian Zhang
human	IF	Science; The mechanism of acentrosomal spindle assembly in human oocytes; Authors - Tianyu Wu
human	IF	Nat Commun; An interaction network of inner centriole proteins organised by POC1A-POC1B heterodimer crosslinks ensures centriolar integrity; Authors - Cornelia Sala

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