Anticorps Polyclonal de lapin anti-HBB

HBB Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 16216-1-AP

Synonymes

beta globin, CD113t C, HBB, Hemoglobin beta chain, Hemoglobin subunit beta, hemoglobin, beta

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Galerie de données de validation

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  WB analysis of human heart using 16216-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 16216-1-AP (HBB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 16216-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 16216-1-AP (HBB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 16216-1-AP

  IP Result of anti-HBB (IP:16216-1-AP, 4ug; Detection:16216-1-AP 1:1000) with mouse heart tissue lysate 3200ug.

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  IHC staining of human liver using 16216-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16216-1-AP (HBB antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 16216-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16216-1-AP (HBB antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of human appendicitis using 16216-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using HBB antibody (16216-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of K-562 using 16216-1-AP

  1X10^6 K-562 cells were intracellularly stained with 0.8 ug Anti-Human HBB (16216-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu cardiaque humain, tissu placentaire humain
• Résultats positifs en IHC: tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu d'appendicite humain,
• Résultats positifs en cytométrie: cellules K-562,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 3 publications below

Informations sur le produit

16216-1-AP cible HBB dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HBB Protéine recombinante Ag9216
• Nom complet: hemoglobin, beta
• Masse moléculaire calculée: 147 aa, 16 kDa
• Poids moléculaire observé: 13-16 kDa
• Numéro d’acquisition GenBank: BC007075
• Symbole du gène: HBB
• Identification du gène (NCBI): 3043
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The hemoglobin molecule is a tetramer consisting of two alpha- and two beta-globin-like chains. HBB gene encodes hemoglobin beta chain. The hemoglobin chains, each with its own heme moiety, cooperate in binding and release of oxygen. Hemoglobin monomers have been found in blood and other tissues including macrophages, alveolar epithelial cells and brain. Defects in HBB have been linked to diseases including Sickle cell anemia, Beta-thalassemia, and Heinz body anemias. This antibody, raised against the full-length human HBB, recognizes the 13-16 kDa HBB in western blot analyses of human heart and placenta.

Publications

Species	Application	Title
rat	WB	Nat Commun; Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model; Authors - Mengyan Hu
mouse	WB,IHC	Elife; ARID1A loss in adult hepatocytes activates β-catenin-mediated erythropoietin transcription.; Authors - Rozenn Riou
human	WB	Stem Cells; Notch and Aryl Hydrocarbon Receptor Signaling Impact Definitive Hematopoiesis from Human Pluripotent Stem Cells.; Authors - Amy Leung
		Sci Rep; EphrinB2 regulates the emergence of a hemogenic endothelium from the aorta.; Authors - Inn-Inn Chen
mouse	IHC	J Comp Neurol; Hemovasculogenic origin of blood vessels in the developing mouse brain.; Authors - Miguel A Gama Sosa
human	WB	Blood Cells Mol Dis; A long noncoding RNA from the HBS1L-MYB intergenic region on chr6q23 regulates human fetal hemoglobin expression.; Authors - Tasha A Morrison

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