Anticorps Polyclonal de lapin anti-Hemoglobin Epsilon

Hemoglobin Epsilon Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 12361-1-AP

Synonymes

HBE1, Epsilon globin, Epsilon-globin, HBE, Hemoglobin epsilon chain

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Galerie de données de validation

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  WB analysis of human placenta using 12361-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12361-1-AP (Hemoglobin epsilon antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse placenta using 12361-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 12361-1-AP (Hemoglobin epsilon antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 12361-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12361-1-AP (Hemoglobin epsilon antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 12361-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12361-1-AP (Hemoglobin epsilon antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 12361-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 12361-1-AP (Hemoglobin epsilon antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 12361-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 12361-1-AP (Hemoglobin epsilon antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of human placenta using 12361-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Hemoglobin Epsilon antibody (12361-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human placenta using 12361-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Hemoglobin Epsilon antibody (12361-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of K-562 using 12361-1-AP

  1x10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human Hemoglobin Epsilon (12361-1-AP)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu placentaire humain, cellules K-562, tissu placentaire de souris
• Résultats positifs en IHC: tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu placentaire humain,
• Résultats positifs en FC (Intra): cellules K-562

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 1 publications below

Informations sur le produit

12361-1-AP cible Hemoglobin Epsilon dans les applications de WB, IHC, IF-P, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Hemoglobin Epsilon Protéine recombinante Ag3026
• Nom complet: hemoglobin, epsilon 1
• Masse moléculaire calculée: 147 aa, 16 kDa
• Poids moléculaire observé: 12-16 kDa
• Numéro d’acquisition GenBank: BC015537
• Symbole du gène: HBE1
• Identification du gène (NCBI): 3046
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The hemoglobin molecule is a tetramer consisting of two alpha- and two beta-globin-like chains. HBE1 (hemoglobin epsilon chain) is a beta-type chain of hemoglobin predominantly expressed during the embryonic stage (21321359). The HBE1 has been regarded as the best marker for fetal nucleated red blood cells (NRBCs) . This antibody can be used to label and isolate fetal cells from maternal blood and may be useful in prenatal diagnosis (15906407).

Publications

Species	Application	Title
human	WB	Blood; NFI-A directs the fate of hematopoietic progenitors to the erythroid or granulocytic lineage and controls beta-globin and G-CSF receptor expression.; Authors - Linda Marie Starnes
human	WB	Cell Death Differ; Transcriptional fine-tuning of microRNA-223 levels directs lineage choice of human hematopoietic progenitors.; Authors - L Vian
human	WB	Mol Cell Biol; A Feedback Loop Consisting of MicroRNA 23a/27a and the β-Like Globin Suppressors KLF3 and SP1 Regulates Globin Gene Expression.; Authors - Ma Yanni Y
human	WB	Biochim Biophys Acta Mol Cell Res; Long noncoding RNA CCDC26 as a modulator of transcriptional switching between fetal and embryonic globins.; Authors - Tetsuo Hirano
rat	WB	Proteome Sci; A comparison of E15.5 fetus and newborn rat serum proteomes.; Authors - Wei Lilong L
mouse	IHC	Blood; Requirement for antiapoptotic MCL-1 during early erythropoiesis; Authors - Meghan E Turnis

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