Anticorps Polyclonal de lapin anti-Hemoglobin Epsilon

Hemoglobin Epsilon Polyclonal Antibody for FC, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, FC, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 12361-1-AP

Synonymes

Epsilon globin, HBE, HBE1, Hemoglobin epsilon chain, Hemoglobin subunit epsilon, hemoglobin, epsilon 1

---

Galerie de données de validation

• 

  WB analysis of human placenta using 12361-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12361-1-AP (Hemoglobin epsilon antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 12361-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12361-1-AP (Hemoglobin epsilon antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse placenta using 12361-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 12361-1-AP (Hemoglobin epsilon antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 12361-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12361-1-AP (Hemoglobin epsilon antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 12361-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 12361-1-AP (Hemoglobin epsilon antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 12361-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 12361-1-AP (Hemoglobin epsilon antibody) at dilution of 1:50 (under 40x lens).

• 

  FC experiment of K-562 using 12361-1-AP

  1X10^6 K-562 cells were stained with 0.2ug Hemoglobin epsilon antibody (12361-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

---

Applications testées

• Résultats positifs en WB: tissu placentaire humain, cellules K-562, tissu placentaire de souris
• Résultats positifs en IHC: tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules K-562

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 1 publications below

Informations sur le produit

12361-1-AP cible Hemoglobin Epsilon dans les applications de WB, IHC, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Hemoglobin Epsilon Protéine recombinante Ag3026
• Nom complet: hemoglobin, epsilon 1
• Masse moléculaire calculée: 147 aa, 16 kDa
• Poids moléculaire observé: 12-16 kDa
• Numéro d’acquisition GenBank: BC015537
• Symbole du gène: HBE1
• Identification du gène (NCBI): 3046
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The hemoglobin molecule is a tetramer consisting of two alpha- and two beta-globin-like chains. HBE1 (hemoglobin epsilon chain) is a beta-type chain of hemoglobin predominantly expressed during the embryonic stage (21321359). The HBE1 has been regarded as the best marker for fetal nucleated red blood cells (NRBCs) . This antibody can be used to label and isolate fetal cells from maternal blood and may be useful in prenatal diagnosis (15906407).

Publications

Species	Application	Title
human	WB	Blood; NFI-A directs the fate of hematopoietic progenitors to the erythroid or granulocytic lineage and controls beta-globin and G-CSF receptor expression.; Authors - Linda Marie Starnes
human	WB	Cell Death Differ; Transcriptional fine-tuning of microRNA-223 levels directs lineage choice of human hematopoietic progenitors.; Authors - L Vian
human	WB	Mol Cell Biol; A Feedback Loop Consisting of MicroRNA 23a/27a and the β-Like Globin Suppressors KLF3 and SP1 Regulates Globin Gene Expression.; Authors - Ma Yanni Y
human	WB	Biochim Biophys Acta Mol Cell Res; Long noncoding RNA CCDC26 as a modulator of transcriptional switching between fetal and embryonic globins.; Authors - Tetsuo Hirano
rat	WB	Proteome Sci; A comparison of E15.5 fetus and newborn rat serum proteomes.; Authors - Wei Lilong L
mouse	IHC	Blood; Requirement for antiapoptotic MCL-1 during early erythropoiesis; Authors - Meghan E Turnis

Produits associés

HBE1-Specific Monoclonal antibody

66151-1-Ig

HBE1-Specific Polyclonal antibody

19994-1-AP

Hemoglobin Alpha Polyclonal antibody

14537-1-AP

HBG1/2-Specific Monoclonal antibody

66168-1-Ig

HBZ Polyclonal antibody

17284-1-AP