Anticorps Polyclonal de lapin anti-HBG1/2-Specific

HBG1/2-Specific Polyclonal Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

N° de cat : 19993-1-AP

Synonymes

HBG1, Gamma 1 globin, Gamma-1-globin, Hb F Agamma, HBGA

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Galerie de données de validation

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  WB analysis of human placenta using 19993-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 19993-1-AP (HBG1/2-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of recombinant proein using 19993-1-AP

  WB results of 19993-1-AP (HBG1/2-Specific antibody) at dilution of 1:2000.

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  FC experiment of K-562 using 19993-1-AP

  1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human HBG1/2-Specific (19993-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu placentaire humain, protéine recombinante
• Résultats positifs en FC (Intra): cellules K-562,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

19993-1-AP cible HBG1/2-Specific dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: hemoglobin, gamma A
• Masse moléculaire calculée: 16 kDa
• Poids moléculaire observé: 16 kDa
• Numéro d’acquisition GenBank: NM_000559
• Symbole du gène: HBG1
• Identification du gène (NCBI): 3047
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HBG1, also named as Hb F Agamma, HBGA, HBGR and HSGGL, belongs to the globin family. HBG1 makes up the fetal hemoglobin F, in combination with alpha chains. Some gamma variants can cause severe jaundice and cyanosis in premature and new born babies. The antibody is specific to HBG1 and HBG2. The antibody has no cross reaction to HBB and HBD. HBG1 protein migrates around 16 kDa. Heterodimer of 32 kDa may also be observed. This antibody could identify HBG1 and HBG2.

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