Anticorps Polyclonal de lapin anti-HDGF

• Phare
• Validé par KD/KO

HDGF Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IHC, IF, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

20

N° de cat : 11344-1-AP

Synonymes

HDGF, Hepatoma derived growth factor, HMG 1L2, HMG1L2

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Galerie de données de validation

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  WB analysis using 11344-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11344-1-AP (HDGF antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 11344-1-AP

  IP Result of anti-HDGF (IP:11344-1-AP, 3ug; Detection:11344-1-AP 1:1000) with HepG2 cells lysate 3000ug.

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  IHC staining of human lung cancer using 11344-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11344-1-AP (HDGF antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung cancer using 11344-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11344-1-AP (HDGF antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary tumor using 11344-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11344-1-AP (HDGF antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary tumor using 11344-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11344-1-AP (HDGF antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon cancer using 11344-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11344-1-AP (HDGF antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon cancer using 11344-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11344-1-AP (HDGF antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver cancer using 11344-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 11344-1-AP (HDGF antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver cancer using 11344-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 11344-1-AP (HDGF antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung cancer using 11344-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11344-1-AP (HDGF antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung cancer using 11344-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11344-1-AP (HDGF antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HEK-293 using 11344-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using HDGF antibody (11344-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HEK-293T, cellules HeLa
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer du côlon humain, tissu de cancer du foie humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 13 publications below
• IHC: See 8 publications below
• IF: See 4 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

11344-1-AP cible HDGF dans les applications de WB, IP, IHC, IF, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HDGF Protéine recombinante Ag1893
• Nom complet: hepatoma-derived growth factor (high-mobility group protein 1-like)
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 40-45 kDa
• Numéro d’acquisition GenBank: BC018991
• Symbole du gène: HDGF
• Identification du gène (NCBI): 3068
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HDGF, also named as HMG1L2, belongs to the HDGF family. HDGF is a mitogen for many cell types with nuclear localization necessary for its mitogenic activity. HDGF has also been identified as an important prognostic marker in pathologic cell growth, as it is overexpressed in a number of cancers with expression linked to a poor outcome in lung, esophageal, pancreatic and hepatic cancer.HDGF is highly expressed in human NSCLC tissues, predicting worse prognosis in resected NSCLCs. It might be useful molecular biomarker for predicting the prognosis of resected NSCLCs.

Publications

Species	Application	Title
human	WB	J Hazard Mater; Proteomic analysis of cell proliferation in a human hepatic cell line (HL-7702) induced by perfluorooctane sulfonate using iTRAQ.; Authors - Ruina Cui
human	WB,IHC,IF,ChIP	Mol Oncol; Role of hepatoma-derived growth factor in promoting de novo lipogenesis and tumorigenesis in hepatocellular carcinoma.; Authors - Xuejie Min ; KD Validated
human	WB	Front Cell Dev Biol; NAP1L1 Functions as a Tumor Promoter via Recruiting Hepatoma-Derived Growth Factor/c-Jun Signal in Hepatocellular Carcinoma.; Authors - Ye-Wei Zhang
human	WB	Eur J Cancer; Identification of glia maturation factor beta as an independent prognostic predictor for serous ovarian cancer.; Authors - Li Yan Li YL
human	WB	Hum Cell; LncRNA SNHG3 promotes cell proliferation and invasion through the miR-384/hepatoma-derived growth factor axis in breast cancer.; Authors - Qiuhong Ma ; KD Validated
human	WB,IF,IP	Cancer Cell Int; NAP1L1 interacts with hepatoma-derived growth factor to recruit c-Jun inducing breast cancer growth.; Authors - Shu Liu