Anticorps Monoclonal anti-HE4

HE4 Monoclonal Antibody for IF/ICC

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

IF/ICC

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

5B2D9

N° de cat : CL594-66557

Synonymes

WFDC2, WAP5, WAP four-disulfide core domain protein 2, WAP four disulfide core domain protein 2, Major epididymis-specific protein E4

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Galerie de données de validation

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  IF Staining of SKOV-3 using CL594-66557

  Immunofluorescent analysis of (-20°C Ethanol) fixed SKOV-3 cells using CoraLite®594 HE4 antibody (CL594-66557, Clone: 5B2D9 ) at dilution of 1:200.

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  IF Staining of SKOV-3 using CL594-66557

  Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using CoraLite®®594 HE4 antibody (CL594-66557, Clone: 5B2D9 ) at dilution of 1:200.

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  FC experiment of OVCAR-3 using CL594-66557

  1x10^6 OVCAR-3 cells were intracellularly stained with 0.4 ug CoraLite®594-conjugated HE4 Monoclonal antibody (CL594-66557, Clone:5B2D9)(red), or 0.4 ug CoraLite®594 Mouse IgG1 Isotype Control (1F8D3) (CL594-66360-1, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of SKOV-3 using CL594-66557

  1x10^6 SKOV-3 cells were intracellularly stained with 0.4 ug CoraLite®594-conjugated HE4 Monoclonal antibody (CL594-66557, Clone:5B2D9)(red), or 0.4 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF/ICC: cellules SKOV-3,
• Résultats positifs en FC (Intra): cellules SKOV-3,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-66557 cible HE4 dans les applications de IF/ICC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HE4 Protéine recombinante Ag5936
• Nom complet: WAP four-disulfide core domain 2
• Masse moléculaire calculée: 13 kDa
• Numéro d’acquisition GenBank: BC046106
• Symbole du gène: HE4
• Identification du gène (NCBI): 10406
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

WFDC2, also named as HE4, is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. HE4 is expressed in a number of normal tissues, and it is also highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. In some study, HE4 was referred to as a biomarker for ovarian carcinoma.

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