Anticorps Polyclonal de lapin anti-HEXB

• Phare
• Validé par KD/KO

HEXB Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 16229-1-AP

Synonymes

ENC 1AS, HCC 7, HEXB, Hexosaminidase subunit B

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Galerie de données de validation

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  WB analysis using 16229-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16229-1-AP (HEXB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16229-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16229-1-AP (HEXB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 16229-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16229-1-AP (HEXB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 16229-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16229-1-AP (HEXB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 16229-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16229-1-AP (HEXB antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 16229-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16229-1-AP (HEXB antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 16229-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16229-1-AP (HEXB antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 16229-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16229-1-AP (HEXB antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules Jurkat, tissu pulmonaire de souris, tissu rénal de rat, tissu rénal de souris
• Résultats positifs en IHC: tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

16229-1-AP cible HEXB dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HEXB Protéine recombinante Ag8683
• Nom complet: hexosaminidase B (beta polypeptide)
• Masse moléculaire calculée: 556 aa, 63 kDa
• Poids moléculaire observé: 63-67 kDa
• Numéro d’acquisition GenBank: BC017378
• Symbole du gène: HEXB
• Identification du gène (NCBI): 3074
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The HEXB (Beta-hexosaminidase subunit beta) gene encodes the beta subunit of the enzyme hexosaminidase, which is involved in the breakdown of gangliosides. It is responsible for the degradation of GM2 gangliosides, and has a variety of other molecules containing terminal N-acetyl hexosamines, in the brain and other tissues. Refer to the domains of HEXB and glycosylation, there will be some different bands with MW 63-67 kDa precursor; 59-63 kDa signal peptide removed; 50-55 kDa subunit beta (or signal and propeptide removed); 20-25 kDa subunit beta chain B; ~30 kDa subunit beta chain A (Uniprot).

Publications

Species	Application	Title
mouse	WB	Mol Neurobiol; The Role of Oxidized Cholesterol in Diabetes-Induced Lysosomal Dysfunction in the Brain.; Authors - Catrina Sims-Robinson
human	WB,IHC	Front Cell Dev Biol; Identification and Validation of a Nine-Gene Amino Acid Metabolism-Related Risk Signature in HCC.; Authors - Yajuan Zhao
human	IHC,IF,WB	Front Oncol; Microglia-Specific Expression of HEXA and HEXB Leads to Poor Prognosis in Glioblastoma Patients.; Authors - Mengxian Jia ; KD Validated
mouse	WB	Biol Open; Evidence for lysosomal exocytosis and release of aggrecan-degrading hydrolases from hypertrophic chondrocytes, in vitro and in vivo.; Authors - Bastow Edward R ER
human	WB	Nat Commun; Deficiency of the frontotemporal dementia gene GRN results in gangliosidosis; Authors - Sebastian Boland
mouse	IF	J Cereb Blood Flow Metab; Characteristics of activation of monocyte-derived macrophages versus microglia after mouse experimental intracerebral hemorrhage; Authors - Fenghui Ye